Recent Chemical Biology Approaches for Profiling Cell Surface Sialylation Status.
Recent Chemical Biology Approaches for Profiling Cell Surface Sialylation Status.
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最近用于分析细胞表面唾液酸化状态的化学生物学方法。
DOI：
10.1021/acschembio.8b00456
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发表时间：
2018-07
影响因子：
4
通讯作者：
Sun Xue Long
中科院分区：
文献类型：
--
作者：
Whited Joshua;Zhang Xiaoqing;Nie Huan;Wang Dan;Li Yu;Sun Xue Long
唾液酸（SA）通常作为细胞表面上的糖蛋白或糖脂的聚糖的末端糖存在，因此直接参与生物过程，例如细胞-细胞、细胞-配体和细胞-病原体相互作用。细胞表面SA表达水平及其连接统称为细胞表面唾液酸化状态，其代表不同的细胞状态并有助于细胞的整体功能。因此，细胞表面唾液酸化状态的系统性和特异性分析在破译细胞表面糖缀合物的结构和功能及其潜在生物过程的分子机制中是至关重要的。近几十年来，已经开发了几种先进的化学生物学方法来分析体外和体内样品的细胞表面唾液酸化状态，包括代谢标记、直接化学修饰和硼酸偶联方法。各种研究技术也因其独特的能力而被探索，包括荧光成像、流式细胞术、拉曼成像、磁共振成像（MRI）和基质辅助激光解吸电离成像质谱。特别地，已经研究了细胞表面上特定糖蛋白的唾液酸化状态。这篇综述强调了最近的进展，在化学生物学方法分析细胞表面唾液酸化状态。预计这篇综述将为生物学和生物医学研究和应用提供不同的选择。
Sialic acids (SAs) often exist as the terminal sugars of glycans of either glycoproteins or glycolipids on the cell surface and thus are directly involved in biological processes, such as cell-cell, cell-ligand, and cell-pathogen interactions. Cell surface SA expression levels and their linkages are collectively termed cell surface sialylation status, which represent varying cellular states and contribute to the overall functionality of a cell. Accordingly, systemic and specific profiling of the cell surface sialyation status is critical in deciphering the structures and functions of cell surface glycoconjugates and the molecular mechanisms of their underlying biological processes. In recent decades, several advanced chemical biology approaches have been developed to profile the cell surface sialyation status of both in vitro and in vivo samples, including metabolic labeling, direct chemical modification, and boronic acid coupling approaches. Various investigative technologies have also been explored for their unique competence, including fluorescent imaging, flow cytometry, Raman imaging, magnetic resonance imaging (MRI), and matrix-assisted laser desorption ionization imaging mass spectrometry. In particular, the sialylation status of a specific glycoprotein on the cell surface has been investigated. This review highlights the recent advancements in chemical biology approaches for profiling cell surface sialyation status. It is expected that this review will provide researchers different choices for both biological and biomedical research and applications.
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影响因子：
16.6
作者：
Chang, Pamela V.;Chen, Xing;Smyrniotis, Chris;Xenakis, Alexander;Hu, Tianshun;Bertozzi, Carolyn R.;Wu, Peng
通讯作者：
Wu, Peng
通过MALDIMIMIGAGE质谱法对肝细胞癌组织的二维N-聚糖分布图。
影响因子：
5.5
作者：
Powers TW;Holst S;Wuhrer M;Mehta AS;Drake RR
通讯作者：
Drake RR
影响因子：
4.3
作者：
E. Roffman;M. Wilchek
通讯作者：
E. Roffman;M. Wilchek
影响因子：
8.8
作者：
Langereis MA;Bakkers MJ;Deng L;Padler-Karavani V;Vervoort SJ;Hulswit RJ;van Vliet AL;Gerwig GJ;de Poot SA;Boot W;van Ederen AM;Heesters BA;van der Loos CM;van Kuppeveld FJ;Yu H;Huizinga EG;Chen X;Varki A;Kamerling JP;de Groot RJ
通讯作者：
de Groot RJ
影响因子：
7.8
作者：
Wang, Zhiyun;Du, Jian;Che, Pao-Lin;Meledeo, M. Adam;Yarema, Kevin J.
通讯作者：
Yarema, Kevin J.