A draft physical map of a D-genome cotton species (Gossypium raimondii).
A draft physical map of a D-genome cotton species (Gossypium raimondii).
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DOI：
10.1186/1471-2164-11-395
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发表时间：
2010-06-22
期刊：
影响因子：
4.4
通讯作者：
Paterson AH
中科院分区：
文献类型：
--
作者：
Lin L;Pierce GJ;Bowers JE;Estill JC;Compton RO;Rainville LK;Kim C;Lemke C;Rong J;Tang H;Wang X;Braidotti M;Chen AH;Chicola K;Collura K;Epps E;Golser W;Grover C;Ingles J;Karunakaran S;Kudrna D;Olive J;Tabassum N;Um E;Wissotski M;Yu Y;Zuccolo A;ur Rahman M;Peterson DG;Wing RA;Wendel JF;Paterson AH
大型真核生物基因组的遗传锚定物理图谱已被证明是有用的，因为它们的内在优点和作为基因组测序的辅助手段。栽培四倍体棉花、陆地棉和棉棉。Barbadense，有一个共同的祖先，大约在1-2百万年前由A和D基因组合并而成。为了表征棉花基因组多样性的长期目标，世界范围内的棉花社区优先考虑D基因组祖先Gossypium raimondii进行完整测序。构建了G.将四倍体棉花的D基因组祖先种雷蒙（raimondii）整合到基因锚定的overgo杂交探针、基于琼脂糖的指纹和“高信息含量指纹”（HICF）中。共使用13，662个BAC末端序列和2，828个DNA探针，将1585个重叠群遗传锚定到棉花共有遗传图谱，并将370个和438个重叠群分别锚定到拟南芥（AT）和葡萄属葡萄（VV）全基因组序列。几条线索的证据表明，G。雷蒙迪基因组由两种性质不同的组分组成。大部分基因丰富的组成部分是对齐的拟南芥和葡萄属葡萄基因组，并显示出利用翻译基因组学方法在理解这一重要的基因组及其居民基因的希望。整合的遗传-物理图谱在组装和验证计划的参考序列中都是有价值的。
Genetically anchored physical maps of large eukaryotic genomes have proven useful both for their intrinsic merit and as an adjunct to genome sequencing. Cultivated tetraploid cottons, Gossypium hirsutum and G. barbadense, share a common ancestor formed by a merger of the A and D genomes about 1-2 million years ago. Toward the long-term goal of characterizing the spectrum of diversity among cotton genomes, the worldwide cotton community has prioritized the D genome progenitor Gossypium raimondii for complete sequencing. A whole genome physical map of G. raimondii, the putative D genome ancestral species of tetraploid cottons was assembled, integrating genetically-anchored overgo hybridization probes, agarose based fingerprints and 'high information content fingerprinting' (HICF). A total of 13,662 BAC-end sequences and 2,828 DNA probes were used in genetically anchoring 1585 contigs to a cotton consensus genetic map, and 370 and 438 contigs, respectively to Arabidopsis thaliana (AT) and Vitis vinifera (VV) whole genome sequences. Several lines of evidence suggest that the G. raimondii genome is comprised of two qualitatively different components. Much of the gene rich component is aligned to the Arabidopsis and Vitis vinifera genomes and shows promise for utilizing translational genomic approaches in understanding this important genome and its resident genes. The integrated genetic-physical map is of value both in assembling and validating a planned reference sequence.
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影响因子：
5.4
作者：
Draye, X;Chee, P;Paterson, AH
通讯作者：
Paterson, AH
影响因子：
4.4
作者：
Cai, WW;Reneker, J;Bradley, A
通讯作者：
Bradley, A
影响因子：
4.4
作者：
Abdurakhmonov, I. Y.;Kohel, R. J.;Abdukarimov, A.
通讯作者：
Abdukarimov, A.
DOI：
10.1073/pnas.0502365102
发表时间：
2005-09-13
影响因子：
11.1
作者：
Bowers, JE;Arias, MA;Paterson, AH
通讯作者：
Paterson, AH
影响因子：
--
作者：
Guo, WZ;Zhang, TZ;Kohel, RJ
通讯作者：
Kohel, RJ