Genistein induces non-rapid eye movement sleep in mice
Genistein induces non-rapid eye movement sleep in mice
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金雀异黄素诱导小鼠非快速动眼睡眠
DOI：
10.1111/j.1479-8425.2012.00571.x
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Qu WM
中科院分区：
文献类型：
--
作者：
Huo XJ;Liu W;Qiu MH,;Huang ZL;Qu WM
长期以来，人们一直认为，治疗核心焦虑症状将产生令人满意的失眠解决方案。金雀异黄素是大豆中的主要异黄酮类成分，已有报道在大鼠身上发挥类似焦虑的作用。然而，金雀异黄素是否调节睡眠行为仍有待阐明。在本研究中，我们通过脑电和肌电记录以及c-Fos的免疫组织化学染色，观察了染料木素对自由活动小鼠睡眠觉醒的影响。我们的结果表明，金雀异黄素腹腔注射。在80和160 mg/kg剂量下，显著增加了非快速眼动(Non-REMNREM)睡眠的数量和NREM睡眠的次数，同时伴随着觉醒的减少。它增加了从清醒到NREM睡眠的状态转换，然后从NREM睡眠到清醒状态的转换。然而，金雀异黄素对REM睡眠量和NREM或REM睡眠的脑电功率密度都没有影响。免疫组织化学染色显示，给予染料木素后，蓝斑和结节乳头核神经元中c-Fos的表达较溶剂对照组减少。这些结果表明，金雀异黄素可抑制促进觉醒的神经元，从而促进NREM睡眠，提示其在治疗失眠方面具有潜在的应用价值。
It has long been thought that the treatment of core anxiety symptoms will produce a satisfactory resolution of insomnia. Genistein, the principal isoflavone constituent of soybean, has been reported to exert anxiolytic-like effects in rats. Whether genistein modulates sleep behavior, however, remains to be elucidated. In the present study, we examined the effect of genistein on sleep wake profiles in freely moving mice by EEG and EMG recording as well as immunohistochemical staining for c-Fos. Our results showed that genistein administered i.p. at doses of 80 and 160 mg/kg significantly increased the amount of non-rapid eye movement (non-REMNREM) sleep and as well as the number of NREM sleep episodes, with a concomitant reduction of wakefulness. It increased the state transitions from wakefulness to NREM sleep and subsequently from NREM to wakefulness. However, genistein neither had an effect on the amount of REM sleep nor on EEG power density of NREM or REM sleep. Immunostaining revealed a reduction of c-Fos expression in neurons of the locus coeruleus and the tuberomammillary nucleus after administration of genistein, as compared with the vehicle control. These results indicate that treatment with genistein leads to an inhibition of arousal-promoting neurons and thus promotes NREM sleep, suggesting its potential application for the treatment of insomnia.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
1.1
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影响因子：
5
作者：
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通讯作者：
A. Pawlyk;P. Alfinito;D. Deecher
影响因子：
25
作者：
Huang, ZL;Qu, WM;Hayaishi, O
通讯作者：
Hayaishi, O