Expression of chemokine receptors CXCR4 and CCR5 in HIV-1-infected and uninfected individuals.
Expression of chemokine receptors CXCR4 and CCR5 in HIV-1-infected and uninfected individuals.
复制标题
HIV-1 感染和未感染个体中趋化因子受体 CXCR4 和 CCR5 的表达。
作者：
M. Ostrowski;S. Justement;A. Catanzaro;Claire A. Hallahan;L. Ehler;S. Mizell;Princy N. Kumar;J. Mican;T. Chun;A. Fauci
趋化因子受体CXCR4和CCR5是HIV-1进入CD4+T细胞的主要辅助受体。在早期疾病中，大多数HIV-1的初级分离株使用CCR5作为辅助受体，而在随着合胞体诱导病毒的出现而出现的疾病进展过程中，也使用CXCR4。我们进行了一项横断面研究，评估了两种HIV-1辅助受体CCR5和CXCR4在HIV-1感染者和未感染者的全血样本中的表达。与未感染对照组相比，HIV感染者外周血中CD8+T细胞、CD14+单核细胞和CD4+T细胞表面CXCR4的表达显著下调，而CD4+T细胞上CCR5的表达显著上调。在HIV感染者和未感染者中，共受体的表达与体内细胞激活水平有关，CXCR4主要表达在静止的(HLA-DR-)T细胞上，而CCR5主要表达在激活的(HLA-DR+)T细胞上。CD4+T细胞上CXCR4低表达和CCR5高表达与疾病进展有关。此外，在晚期疾病患者中，观察到CXCR4+CD4+T细胞有更大的激活趋势。然而，根据CD4+T细胞活化水平或趋化因子受体的表达水平，无法区分携带合胞体诱导病毒的患者和携带非合胞体诱导病毒的患者。
The chemokine receptors CXCR4 and CCR5 have been identified as major coreceptors for HIV-1 entry into CD4+ T cells. The majority of primary HIV-1 isolates in early disease use CCR5 as a coreceptor, whereas during disease progression with the emergence of syncytium-inducing viruses, CXCR4 is also used. We performed a cross-sectional study in which we evaluated the expression of two HIV-1 coreceptors, CCR5 and CXCR4, in whole blood samples taken from HIV-1-infected and uninfected individuals. We demonstrate that CXCR4 on CD4+ and CD8+ T cells, and CD14+ monocytes is significantly down-regulated, and CCR5 expression on CD4+ T cells is up-regulated in HIV-infected individuals compared with uninfected controls. Coreceptor expression correlated with the level of cellular activation in vivo in both HIV-infected and uninfected individuals, with CXCR4 being expressed predominantly on quiescent (HLA-DR-) T cells and CCR5 being expressed predominantly on activated (HLA-DR+) T cells. Lower expression of CXCR4 and higher expression of CCR5 on CD4+ T cells correlated with advancing disease. In addition, a tendency for greater activation of CXCR4+CD4+ T cells in patients with advanced disease was observed. Patients who harbored syncytium-inducing viruses, however, could not be distinguished from those who harbored nonsyncytium-inducing viruses based on the level of CD4+ T cell activation or chemokine receptor expression.
影响因子：
6.4
作者：
GIORGI, JV;HO, HN;SEMINARA, D
通讯作者：
SEMINARA, D
DOI：
10.1073/pnas.94.5.1925
发表时间：
1997-03-04
影响因子：
11.1
作者：
Bleul, CC;Wu, LJ;Mackay, CR
通讯作者：
Mackay, CR
影响因子：
56.9
作者：
ZHU, TF;MO, HM;HO, DD
通讯作者：
HO, DD