Inverse relationship between pilus-mediated gonococcal adherence and surface expression of the pilus receptor, CD46.
Inverse relationship between pilus-mediated gonococcal adherence and surface expression of the pilus receptor, CD46.
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菌毛介导的淋球菌粘附与菌毛受体 CD46 的表面表达之间呈负相关。
DOI：
10.1099/00221287-147-8-2333
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Seifert,HSteven
中科院分区：
文献类型：
--
作者：
Tobiason,DeborahM;Seifert,HSteven
毛菌介导的粘膜上皮细胞粘附是淋病奈瑟菌在人类宿主中建立感染的关键步骤。CD46是淋球菌菌毛的明确受体，是一种在所有人类有核细胞上表达的互补调节蛋白。结果表明，淋球菌菌株FA1090的毛状Opa -变异株以不同的效率粘附在人上皮细胞系（Chang、ME180、HEC-1B和PC-3）上。令人惊讶的是，这些粘附水平的差异与这些细胞系表达的CD46水平无关。事实上，表面暴露的CD46总量与淋球菌粘附呈反比关系。CD46的四种主要亚型是由于表面暴露区域和细胞质尾部的RNA剪接而产生的。测定了各细胞系的相对异构体表面表达量，发现各细胞系表达4种CD46异构体的比例不同。CD46异构体表面表达与菌毛介导的淋球菌粘附之间没有相关性，表明CD46不作为淋球菌菌毛的经典受体。
Pilus-mediated adherence to mucosal epithelial cells is a critical step forNeisseria gonorrhoeaeto establish an infection in the human host. CD46, the defined receptor for the gonococcal pilus, is a complement-regulatory protein that is expressed on all human nucleated cells. It was observed that a piliated, Opa−variant of gonococcal strain FA1090 adhered with different efficiencies to the human epithelial cell lines tested (Chang, ME180, HEC-1B and PC-3). Surprisingly, these differences in adherence levels did not correlate with levels of CD46 expressed by these cell lines. In fact, there was an inverse relationship between total surface-exposed CD46 and gonococcal adherence. Four major isoforms of CD46 are produced due to alternative RNA splicing of a surface-exposed region and the cytoplasmic tail. The relative isoform surface expression of each cell line was determined, and each was found to express different ratios of the four CD46 isoforms. No correlation could be derived between CD46 isoform surface expression and pilus-mediated gonococcal adherence, indicating that CD46 does not act as a classic receptor for gonococcal pili.
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菌毛介导的淋病奈瑟菌粘附到人上皮细胞所需的两种可变蛋白（PilE 和 PilC）的相互作用
影响因子：
3.6
作者：
T. Rudel;J. Putten;C. Gibbs;R. Haas;T. Meyer
通讯作者：
T. Meyer
DOI：
--
发表时间：
1973
期刊：
Journal of the American Medical Association (JAMA)
影响因子：
--
作者：
H. Handsfield;W. A. Hodson;K. Holmes
通讯作者：
K. Holmes
影响因子：
3.6
作者：
JONSSON, AB;ILVER, D;NORMARK, S
通讯作者：
NORMARK, S
影响因子：
2.2
作者：
Logan, RPH;Robins, A;Hawkey, CJ
通讯作者：
Hawkey, CJ
影响因子：
4.8
作者：
Källström, H;Islam, MS;Jonsson, AB
通讯作者：
Jonsson, AB