Interaction of two variable proteins (PilE and PilC) required for pilus‐mediated adherence of Neisseria gonorrhoeae to human epithelial cells
Interaction of two variable proteins (PilE and PilC) required for pilus‐mediated adherence of Neisseria gonorrhoeae to human epithelial cells
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菌毛介导的淋病奈瑟菌粘附到人上皮细胞所需的两种可变蛋白（PilE 和 PilC）的相互作用
作者：
T. Rudel;J. Putten;C. Gibbs;R. Haas;T. Meyer
皮利赋予淋球菌与上皮细胞结合的初始能力。菌毛蛋白（PilE），主要的菌毛亚基，和一个次要的蛋白质称为PilC，据报道是必不可少的菌毛生物发生，经历菌株内阶段和结构变异。我们在这里证明，至少有两种不同的粘附特性与淋球菌皮利：一个是特定的红细胞，这是几乎不受PilE的变化，另一个是特定的上皮细胞，并调制响应PilE的变化。基于这一发现，选择了recA-菌株的突变体，其丧失了与人角膜上皮细胞结合的能力（A-），但保留了形成皮利（P+）和凝集人红细胞（H+）的能力。粘附阴性突变体不能产生可检测水平的PMC 1或PilC 2蛋白，代表在不存在RecA的情况下产生的pilC相变体。在产生的PilE量及其电泳迁移率、纤毛化和血凝程度以及pilE核苷酸序列方面，A-pilC相变体与其A+亲本和自发A+回复突变体无法区分。这些数据表明PilC在菌毛介导的N.淋球菌对人上皮细胞的作用，并进一步表明PNC 1和PilC 2都不是淋球菌皮利装配的必需品。
Pili confer the initial ability of Neisseria gonorrhoeae to bind to epithelial cells. Pilin (PilE), the major pilus subunit, and a minor protein termed PilC, reportedly essential for pilus biogenesis, undergo intra‐strain phase and structural variation. We demonstrate here that at least two different adherence properties are associated with the gonococcal pili: one is specific for erythrocytes, which is virtually unaffected by PilE variation, and another is specific for epithelial cells, and is modulated in response to the variation of PilE. Based on this finding, mutants of a recA ‐ strain were selected that had lost the ability to bind to human cornea epithelial cells (A‐) but retained the ability to form pili (P+) and to agglutinate human erythrocytes (H+). The adherence‐negative mutants failed to produce detectable levels of PMC1 or PilC2 proteins, representing pilC phase variants generated in the absence of RecA. The A‐pilC phase variants were indistinguishable from their A+parents and spontaneous A+ revertants with regard to the amount of PilE produced and its electrophoretic mobility, the degrees of piliation and haemagglutination, and the pilE nucleotide sequence. These data demonstrate a central role for PilC in pilus‐mediated adherence of N. gonorrhoeae to human epithelial cells and further indicate that neither PNC1 nor PilC2 is obligatory for the assembly of gonococcal pili.
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淋病奈瑟菌中的菌毛蛋白表达受到正转录和负转录控制。
DOI：
10.1002/j.1460-2075.1988.tb03335.x
发表时间：
1988
期刊：
The EMBO journal
影响因子：
--
作者：
Taha,MK;So,M;Seifert,HS;Billyard,E;Marchal,C
通讯作者：
Marchal,C
鉴定可能是淋病奈瑟菌受体的碳水化合物结构。
DOI：
10.1073/pnas.85.13.4902
发表时间：
1988
影响因子：
11.1
作者：
Stromberg,N;Deal,C;Nyberg,G;Normark,S;So,M;Karlsson,KA
通讯作者：
Karlsson,KA
DOI：
10.1073/pnas.81.19.6110
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MEYER, TF;BILLYARD, E;SO, M
通讯作者：
SO, M
影响因子：
56.9
作者：
GIRON, JA;HO, ASY;SCHOOLNIK, GK
通讯作者：
SCHOOLNIK, GK
recA 突变对淋病奈瑟菌菌毛抗原变异和相变的影响。
影响因子：
3.3
作者：
Koomey,M;Gotschlich,EC;Robbins,K;Bergström,S;Swanson,J
通讯作者：
Swanson,J