Effects of the nitric oxide synthase inhibitor NG-nitro-L-arginine on the erectile response to cavernous nerve stimulation in the rabbit.
Effects of the nitric oxide synthase inhibitor NG-nitro-L-arginine on the erectile response to cavernous nerve stimulation in the rabbit.
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一氧化氮合酶抑制剂 NG-硝基-L-精氨酸对兔海绵体神经刺激勃起反应的影响。
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
F. Holmquist;Stief Cg;U. Jonas;K.
采用家兔模型，研究了l -精氨酸/一氧化氮通路在阴茎勃起中的作用。平均基础海绵内压为21 cm H2O。海绵体神经刺激（4-8 V, 20-30 Hz）使压力增加到约130 cm H2O。这种反应是高度可重复的，通常与阴茎完全勃起有关。压力的增加可以量化为：(1)压力增加的初始上升部分的斜率；(2) δ P，定义为增产后获得的最大压力减去增产前的基础压力；(3) T90，定义为达到90% δ p的时间。阴茎内给予l -精氨酸/一氧化氮合成抑制剂ng -硝基- l -精氨酸对全身动脉血压没有影响。然而，通过相同途径给药的ng -硝基- l -精氨酸（0.22和2.19 mg）可消除海绵体神经刺激引起的勃起反应；T90显著升高，斜率和δ P显著降低。而ng -硝基- d -精氨酸（2.19）则无抑制作用。l -精氨酸（21.07 mg）直接给药或在ng -硝基l -精氨酸之后给药对海绵状神经刺激的功能反应没有一致的影响。结果表明，药物诱导对兔海绵内压力的影响可以定量描述，该模型可能有助于研究体内控制阴茎勃起的机制。ng -硝基- l -精氨酸的显著抑制作用表明精氨酸/一氧化氮通路在调节勃起所需的阴茎平滑肌松弛中的重要作用。
Using a rabbit model, the involvement of the L-arginine/nitric oxide pathway in penile erection was investigated. The mean basal intracavernous pressure was 21 cm H2O. Cavernous nerve stimulation (4-8 V, 20-30 Hz) increased the pressure to approximately 130 cm H2O. This response was highly reproducible and usually associated with full penile erection. The pressure increase could be quantified in terms of: (1) the slope of the initial, ascending part of the pressure increase; (2) delta P, which was defined as the maximal pressure obtained by the stimulation minus the basal pressure before the stimulation; (3) T90, which was defined as the time to reach 90 per cent of delta P. Intrapenile administration of the L-arginine/nitric oxide synthesis inhibitor NG-nitro-L-arginine had no effect on systemic arterial blood pressure. However, NG-nitro-L-arginine (0.22 and 2.19 mg), administered via the same route, abolished the erectile response induced by cavernous nerve stimulation; T90 increased and slope and delta P decreased significantly. NG-nitro-D-arginine (2.19), on the other hand, had no inhibitory effect. L-arginine (21.07 mg), given either directly or after NG-nitro-L-arginine had no consistent effect on the functional response to cavernous nerve stimulation. The results suggest that pharmacologically induced effects on intracavernous pressure in the rabbit can be described quantitatively, and that this model may be useful to study the mechanisms controlling penile erection in vivo. The pronounced inhibitory action of NG-nitro-L-arginine demonstrates the important role of the arginine/nitric oxide pathway in mediating relaxation of penile smooth muscles necessary for erection.
动脉 L-精氨酸的消耗导致对内皮依赖性舒张的可逆耐受。
DOI：
10.1016/0006-291x(89)91518-0
发表时间：
1989
影响因子：
3.1
作者：
Gold,ME;Bush,PA;Ignarro,LJ
通讯作者：
Ignarro,LJ
DOI：
10.1016/0006-291x(90)92168-y
发表时间：
1990-07-31
影响因子：
3.1
作者：
IGNARRO, LJ;BUSH, PA;RAJFER, J
通讯作者：
RAJFER, J