Differences in atrial fibrillation‑associated proteins between the left and right atrial appendages from patients with rheumatic mitral valve disease: A comparative proteomic analysis.
Differences in atrial fibrillation‑associated proteins between the left and right atrial appendages from patients with rheumatic mitral valve disease: A comparative proteomic analysis.
复制标题
风湿性二尖瓣疾病患者左右心耳之间房颤相关蛋白的差异：比较蛋白质组学分析
DOI：
10.3892/mmr.2016.5776
复制
发表时间：
2016-11
影响因子：
3.4
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Liu H;Chen G;Zheng H;Qin H;Liang M;Feng K;Wu Z
大多数蛋白质组学研究都集中在鉴定右心房（RA）中的房颤（AF）相关蛋白，因此RA和左心房（LA）之间AF相关蛋白的潜在差异仍然未知。本研究的目的是进行蛋白质组学分析，以比较风湿性二尖瓣疾病（RMVD）患者右心耳（RAA）和左心耳（LAA）之间AF相关蛋白的潜在差异。在二尖瓣置换术期间，从18名RMVD患者（10名AF患者）中获得RAA和LAA组织。使用这些组织进行二维荧光差异凝胶电泳（2-D DIGE）蛋白质组学分析，以鉴定RAA和LAA中的AF相关蛋白。随后，蛋白质组学数据进行了验证，使用蛋白质印迹分析的9个选定的蛋白质。在RAA中，32个AF相关蛋白显著失调（15个上调，17个下调）。在LAA中，31种AF相关蛋白显著失调（13种上调，18种下调）。在这些AF相关蛋白中，17个在RAA和LAA中均与AF相关，15个仅在RAA中与AF相关，14个仅在LAA中与AF相关。在差异表达的蛋白质中，western blot分析验证了6个AF相关蛋白的结果，并证明与2-D DIGE结果相比，RAA和LAA中的分布相似。在这些蛋白质中，2种蛋白质在RAA和LAA中都与AF相关，2种仅在RAA中与AF相关，2种仅在LAA中与AF相关。此外，本研究还分析了房颤相关蛋白在RMVD患者左心耳和右心耳的不同分布，这可能反映了RA和LA在房颤中的不同调节机制。这些发现可能为RMVD患者房颤的分子机制提供新的见解。
The majority of proteomic studies have focused on identifying atrial fibrillation (AF)-associated proteins in the right atrium (RA), thus potential differences in AF-associated proteins between the RA and left atrium (LA) remain unknown. The aim of the present study was to perform proteomic analysis to compare the potential differences in AF-associated proteins between the right atrial appendage (RAA) and left atrial appendage (LAA) in patients with rheumatic mitral valve disease (RMVD). RAA and LAA tissues were obtained from 18 patients with RMVD (10 with AF) during mitral valve replacement surgery. Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) proteomics analysis was performed using these tissues to identify AF-associated proteins in RAA and LAA. Subsequently, the proteomics data was validated using western blot analysis of nine selected proteins. In RAA, 32 AF-associated proteins were significantly dysregulated (15 upregulated and 17 downregulated). In LAA, 31 AF-associated proteins were significantly dysregulated (13 upregulated and 18 downregulated). Among these AF-associated proteins, 17 were AF-associated in both RAA and LAA, 15 were AF-associated only in RAA, and 14 were AF-associated only in LAA. Amongst the differentially expressed proteins, western blot analysis validated the results for 6 AF-associated proteins, and demonstrated similar distributions in RAA and LAA compared with the 2-D DIGE results. Of these proteins, 2 proteins were AF-associated in both RAA and LAA, 2 were AF-associated only in RAA, and 2 were AF-associated only in LAA. Additionally, the different distributions of AF-associated proteins in the RAA and LAA of patients with RMVD was analyzed, which may reflect the different regulatory mechanisms of the RA and LA in AF. These findings may provide new insights into the underlying molecular mechanisms of AF in patients with RMVD.
登录
查看更多内容
影响因子：
24
作者：
Mayr, Manuel;Yusuf, Shamil;Camm, A. John
通讯作者：
Camm, A. John
影响因子：
3.4
作者：
Huang, Wen-Jun;Zhou, Rui;Yang, Jin-Liang
通讯作者：
Yang, Jin-Liang
DOI：
10.1046/j.1540-8167.2004.03423.x
发表时间：
2004-02-01
影响因子：
2.7
作者：
Lai, LP;Lin, JL;Huang, SKS
通讯作者：
Huang, SKS
影响因子：
20.1
作者：
Li, DS;Zhang, LM;Nattel, S
通讯作者：
Nattel, S
通过基于 DIGE 的蛋白质组学分析分析与前列腺癌淋巴结转移相关的蛋白质标记
影响因子：
4.4
作者：
Pang, Jun;Liu, Wei-Peng;Gao, Xin
通讯作者：
Gao, Xin