Heterogeneity of midgut cells and their differential responses to blood meal ingestion by the mosquito, Aedes aegypti.
Heterogeneity of midgut cells and their differential responses to blood meal ingestion by the mosquito, Aedes aegypti.
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中肠细胞的异质性及其对蚊子艾德斯埃及摄入血餐的差异反应。
DOI：
10.1016/j.ibmb.2020.103496
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发表时间：
2020-12
影响因子：
3.8
通讯作者：
Franz AWE
中科院分区：
文献类型：
--
作者：
Cui Y;Franz AWE
蚊子是最臭名昭著的吸血昆虫，由于它们的吸血行为和遗传相容性，许多蚊子物种是某些人类致病寄生虫和病毒的高效载体。蚊虫中肠是消化血粉和吸收营养物质的主要器官。它也是感染血餐获得性寄生虫和病毒的初始部位。我们进行了一项基于单核RNA测序（snRNA-Seq）的分析，以评估中肠的细胞多样性以及单个细胞如何对血餐摄入做出反应以促进其消化。我们的研究揭示了埃及伊蚊雌性中肠中存在20个可区分的细胞类型簇。鉴定的细胞类型包括肠干细胞（ISC）、肠母细胞（EB）、分化型EB（dEB）、肠内分泌细胞（EE）、肠上皮细胞（EC）、EC样细胞、贲门细胞和内脏肌（VM）细胞。血餐摄入显着改变了整体中肠细胞类型的组成，大大增加了ISC和三个EC/EC样集群的比例。此外，所有细胞类型的转录谱受到强烈影响，而参与各种代谢过程的基因显著上调。我们的研究为进一步研究蚊子中肠的血液消化、营养吸收和细胞内稳态提供了基础。
Mosquitoes are the most notorious hematophagous insects and due to their blood feeding behavior and genetic compatibility, numerous mosquito species are highly efficient vectors for certain human pathogenic parasites and viruses. The mosquito midgut is the principal organ of blood meal digestion and nutrient absorption. It is also the initial site of infection with blood meal acquired parasites and viruses. We conducted an analysis based on single-nucleus RNA sequencing (snRNA-Seq) to assess the cellular diversity of the midgut and how individual cells respond to blood meal ingestion to facilitate its digestion. Our study revealed the presence of 20 distinguishable cell-type clusters in the female midgut of Aedes aegypti. The identified cell types included intestinal stem cells (ISC), enteroblasts (EB), differentiating EB (dEB), enteroendocrine cells (EE), enterocytes (EC), EC-like cells, cardia cells, and visceral muscle (VM) cells. Blood meal ingestion dramatically changed the overall midgut cell type composition, profoundly increasing the proportions of ISC and three EC/EC-like clusters. In addition, transcriptional profiles of all cell types were strongly affected while genes involved in various metabolic processes were significantly upregulated. Our study provides a basis for further physiological and molecular studies on blood digestion, nutrient absorption, and cellular homeostasis in the mosquito midgut.
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影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
8.8
作者：
Chung BY;Ro J;Hutter SA;Miller KM;Guduguntla LS;Kondo S;Pletcher SD
通讯作者：
Pletcher SD
影响因子：
3.3
作者：
Cazares-Raga, F. E.;Chavez-Munguia, B.;Hernandez-Hernandez, F. C.
通讯作者：
Hernandez-Hernandez, F. C.
影响因子：
8.8
作者：
Dutta, Devanjali;Dobson, Adam J.;Buchon, Nicolas
通讯作者：
Buchon, Nicolas
影响因子：
5.4
作者：
Holland PW;Booth HA;Bruford EA
通讯作者：
Bruford EA