Cell sourcing for bone tissue engineering: amniotic fluid stem cells have a delayed, robust differentiation compared to mesenchymal stem cells.
Cell sourcing for bone tissue engineering: amniotic fluid stem cells have a delayed, robust differentiation compared to mesenchymal stem cells.
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DOI：
10.1016/j.scr.2011.03.001
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发表时间：
2011-07
影响因子：
1.2
通讯作者：
Guldberg, Robert E.
中科院分区：
文献类型：
--
作者：
Peister, Alexandra;Woodruff, Maria A.;Prince, Jarod J.;Gray, Derwin P.;Hutmacher, Dietmar W.;Guldberg, Robert E.
基于细胞的骨再生疗法是一项令人兴奋的新兴技术，但成骨细胞的可用性有限，并且尚未确定理想的细胞来源。对羊水源性干细胞 (AFS) 和骨髓源性间充质干细胞 (MSC) 进行比较，以确定它们在 2D 和 3D 环境中的成骨分化能力。在 2D 培养中，AFS 细胞产生更多矿化基质，但成骨标记物的峰值延迟。细胞还在聚-ε-己内酯构建的 3D 支架上培养 15 周。 MSC 在 3D 支架上的分化速度比 AFS 细胞更快，但矿化基质的产生在 5 周后显着减慢。相比之下，AFS 细胞矿化率持续增加至 15 周，此时 AFS 构建体含有的矿化基质是 MSC 构建体的 5 倍。因此，在用于细胞治疗时应考虑细胞来源，因为 MSC 是立即基质生产的良好选择，但 AFS 细胞将在较长时间内持续强劲的矿化。这项研究表明，干细胞来源可以显着影响体外成骨分化的程度和速率。
Cell based therapies for bone regeneration are an exciting emerging technology, but the availability of osteogenic cells is limited and an ideal cell source has not been identified. Amniotic fluid-derived stem (AFS) cells and bone-marrow derived mesenchymal stem cells (MSCs) were compared to determine their osteogenic differentiation capacity in both 2D and 3D environments. In 2D culture, the AFS cells produced more mineralized matrix but delayed peaks in osteogenic markers. Cells were also cultured on 3D scaffolds constructed of poly-ε-caprolactone for 15 weeks. MSCs differentiated more quickly than AFS cells on 3D scaffolds, but mineralized matrix production slowed considerably after 5 weeks. In contrast, the rate of AFS cell mineralization continued to increase out to 15 weeks, at which time AFS constructs contained 5-fold more mineralized matrix than MSC constructs. Therefore, cell source should be taken into consideration when used for cell therapy, as the MSCs would be a good choice for immediate matrix production, but the AFS cells would continue robust mineralization for an extended period of time. This study demonstrates that stem cell source can dramatically influence the magnitude and rate of osteogenic differentiation in vitro.
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影响因子：
1
作者：
Peister, Alexandra;Porter, Blaise D.;Guldberg, Robert E.
通讯作者：
Guldberg, Robert E.
影响因子：
6
作者：
Hauser, Peter V.;De Fazio, Roberta;Camussi, Giovanni
通讯作者：
Camussi, Giovanni
影响因子：
4
作者：
Cananzi, Mara;Atala, Anthony;De Coppi, Paolo
通讯作者：
De Coppi, Paolo
影响因子：
6.1
作者：
Prusa, AR;Marton, E;Hengstschläger, M
通讯作者：
Hengstschläger, M
影响因子：
--
作者：
Caplan, AI
通讯作者：
Caplan, AI