Formation and maturation of the native cerebral collateral circulation.
Formation and maturation of the native cerebral collateral circulation.
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DOI：
10.1016/j.yjmcc.2010.03.014
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发表时间：
2010-08
影响因子：
5
通讯作者：
Faber JE
中科院分区：
文献类型：
--
作者：
Chalothorn D;Faber JE
如果发生阻塞性血管疾病，天然（预先存在的）侧支循环可使组织损伤最小化。有证据表明，健康个体之间存在侧支程度的巨大差异，可能来自未知的遗传和/或环境因素。关于何时或如何形成原生侧枝所需的信息来识别这些因素知之甚少。我们研究了BALB/c和C57 BL/6小鼠胚胎品系大脑中动脉和前动脉树之间的侧支发育，成年侧支密度和直径有显著差异（BALB/c少85%，小50%）。循环扩张、固定和染色。到E15.5，两种品系的“初级侧支丛”开始形成。E18.5时，血管丛数量达到高峰，但减少60%，直径变小（P<0.001）。检查较早的时间点，以确定这些差异是否与一般循环模式的差异相关。在~E9.0时，BALB/c各品系间的初级毛细血管丛相似，但到E12.5时，分支数减少，直径增大（P<0.05）。在E12.5-E18.5之间-在软脑膜动脉树发育期间-树大小、分支数量和分支之间的距离的小差异与侧支发生的大差异无关。P1-P21之间的新生侧枝修剪在两个品系中是相当的，产生成年密度，但直径和曲折度在BALB/c中增加较少。尽管BALB/c小鼠中VEGF-A和PDGF-B表达较低，但周细胞向新生侧枝的募集是相当的。这些发现表明，侧枝形成晚于血管发育和出生后成熟，遗传背景的差异对这些过程有显着的影响。
The native (pre-existing) collateral circulation minimizes tissue injury if obstructive vascular disease develops. Evidence suggests large differences in collateral extent exist among healthy individuals, presumably from as-yet unknown genetic and/or environmental factors. Little is known regarding when or how native collaterals form—information needed to identify these factors. We examined collateral development between the middle and anterior cerebral artery trees in BALB/c and C57BL/6 mouse embryos—strains with marked differences in adult collateral density and diameter (85% fewer, 50% smaller in BALB/c). The circulation was dilated, fixed and stained. By E15.5, a “primary collateral plexus” was beginning to form in both strains. By E18.5, plexus vessel number peaked, but was 60% less and diameter smaller in BALB/c (P<0.001). Earlier time-points were examined to determine if these differences correlated with differences in patterning of the general circulation. At ~E9.0, the primary capillary plexus was similar between strains, but by E12.5 branching was less and diameter larger in BALB/c (P<0.05). Between E12.5–E18.5—during pial artery tree development—small differences in tree size, branch number and distance between branches did not correlate with the large difference in collaterogenesis. Pruning of nascent collaterals between P1–P21 was comparable in both strains, yielding the adult density, but diameter and tortuosity increased less in BALB/c. Pericyte recruitment to nascent collaterals was comparable, despite lower VEGF-A and PDGF-B expression in BALB/c mice. These findings demonstrate that collaterals form late during vascular development and undergo postnatal maturation, and that differences in genetic background have dramatic effects on these processes.
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影响因子：
3.3
作者：
Ward, Nicole L.;Moore, Elizabeth;LaManna, Joseph C.
通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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