Three-dimensional analysis of vascular development in the mouse embryo.
Three-dimensional analysis of vascular development in the mouse embryo.
复制标题
小鼠胚胎中血管发育的三维分析。
DOI：
10.1371/journal.pone.0002853
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发表时间：
2008-08-06
期刊：
影响因子：
3.7
通讯作者：
Henkelman, R. Mark
中科院分区：
文献类型：
--
作者：
Walls, Johnathon R.;Coultas, Leigh;Rossant, Janet;Henkelman, R. Mark
关键的血管生成（从头血管形成）和血管生成（血管重塑）事件发生在妊娠第 8.0 和 10.0 胚胎日 (E) 之间的小鼠胚胎中，在此期间，脉管系统从简单的循环回路发育为复杂的、结构精细的三维器官。历史上，对信号通路突变体所表现出的血管表型的解释一直受到无法全面成像形成早期小鼠血管系统基本结构的正常事件序列的阻碍。我们采用光学投影断层扫描 (OPT)，使用基于频率距离关系 (FDR) 的反卷积对用内皮特异性标记物 PECAM-1 免疫染色的胚胎进行成像，以创建 E8.0 和 E10.0（5 至 30 个体节）之间的小鼠血管发育的高分辨率三维图谱。对图谱的分析提供了有关体间血管、神经周围血管丛、头丛以及连接胚胎和胚胎外循环的血管正常发育的重要新信息。我们描述了血管重塑的例子，这些例子为发芽血管生成、血管引导线索和动脉/静脉身份的机制提供了新的见解，这些机制与在影响 E8.0 和 E10.0 之间血管发育的小鼠突变体中观察到的表型直接相关。该图集可在 http://www.mouseimaging.ca/research/mouse_atlas.html 免费获取，并将作为深入了解正常和异常血管发育的平台。
Key vasculogenic (de-novo vessel forming) and angiogenic (vessel remodelling) events occur in the mouse embryo between embryonic days (E) 8.0 and 10.0 of gestation, during which time the vasculature develops from a simple circulatory loop into a complex, fine structured, three-dimensional organ. Interpretation of vascular phenotypes exhibited by signalling pathway mutants has historically been hindered by an inability to comprehensively image the normal sequence of events that shape the basic architecture of the early mouse vascular system. We have employed Optical Projection Tomography (OPT) using frequency distance relationship (FDR)-based deconvolution to image embryos immunostained with the endothelial specific marker PECAM-1 to create a high resolution, three-dimensional atlas of mouse vascular development between E8.0 and E10.0 (5 to 30 somites). Analysis of the atlas has provided significant new information regarding normal development of intersomitic vessels, the perineural vascular plexus, the cephalic plexus and vessels connecting the embryonic and extraembryonic circulation. We describe examples of vascular remodelling that provide new insight into the mechanisms of sprouting angiogenesis, vascular guidance cues and artery/vein identity that directly relate to phenotypes observed in mouse mutants affecting vascular development between E8.0 and E10.0. This atlas is freely available at http://www.mouseimaging.ca/research/mouse_atlas.html and will serve as a platform to provide insight into normal and abnormal vascular development.
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影响因子：
2.7
作者：
Fisher, Malcolm E.;Clelland, Allyson K.;Bain, Andrew;Baldock, Richard A.;Murphy, Paula;Downie, Helen;Tickle, Cheryll;Davidson, Duncan R.;Buckland, Richard A.
通讯作者：
Buckland, Richard A.
影响因子：
2.7
作者：
Ambler, CA;Nowicki, JL;Bautch, VL
通讯作者：
Bautch, VL
影响因子：
2.6
作者：
COULY, G;COLTEY, P;LEDOUARIN, NM
通讯作者：
LEDOUARIN, NM
影响因子：
20.3
作者：
Drake, CJ;Fleming, PA
通讯作者：
Fleming, PA
影响因子：
--
作者：
Eremina, Vera;Baelde, Hans J.;Quaggin, Susan E.
通讯作者：
Quaggin, Susan E.