Identification of Aeromonas hydrophila Genes Preferentially Expressed after Phagocytosis by Tetrahymena and Involvement of Methionine Sulfoxide Reductases.
Identification of Aeromonas hydrophila Genes Preferentially Expressed after Phagocytosis by Tetrahymena and Involvement of Methionine Sulfoxide Reductases.
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DOI：
10.3389/fcimb.2016.00199
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发表时间：
2016
影响因子：
5.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Pang M;Lin X;Liu J;Guo C;Gao S;Du H;Lu C;Liu Y
自由生活的原生动物影响病原体在环境中的生存和毒力进化。在这项研究中，我们探讨了气单胞菌的命运时，共培养的嗜热四膜虫和细菌的基因表达与共培养。毒力A.在这种原生动物的空泡中，发现Escherichia菌株具有逃避消化的能力。以.结果表明，在与T.通过转录序列的选择性捕获（SCOTS）和比较斑点印迹分析，对嗜热菌进行鉴定。这些基因中的大部分（42/116）在代谢中起作用，并且其中一些基因先前已被表征为巨噬细胞内细菌存活和复制所需的基因。然后，我们灭活了A.你好与野生型相比，突变体ΔmsrA和ΔmsrAB对T.在斑马鱼中的50%致死剂量（LD 50）测定表明，两种突变体都是高度减毒的。这项研究为研究细菌防御的机制和意义奠定了坚实的基础。
Free-living protozoa affect the survival and virulence evolution of pathogens in the environment. In this study, we explored the fate of Aeromonas hydrophila when co-cultured with the bacteriovorous ciliate Tetrahymena thermophila and investigated bacterial gene expression associated with the co-culture. Virulent A. hydrophila strains were found to have ability to evade digestion in the vacuoles of this protozoan. In A. hydrophila, a total of 116 genes were identified as up-regulated following co-culture with T. thermophila by selective capture of transcribed sequences (SCOTS) and comparative dot-blot analysis. A large proportion of these genes (42/116) play a role in metabolism, and some of the genes have previously been characterized as required for bacterial survival and replication within macrophages. Then, we inactivated the genes encoding methionine sulfoxide reductases, msrA, and msrB, in A. hydrophila. Compared to the wild-type, the mutants ΔmsrA and ΔmsrAB displayed significantly reduced resistance to predation by T. thermophila, and 50% lethal dose (LD50) determinations in zebrafish demonstrated that both mutants were highly attenuated. This study forms a solid foundation for the study of mechanisms and implications of bacterial defenses.
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影响因子：
3.7
作者：
Guo CM;Chen RR;Kalhoro DH;Wang ZF;Liu GJ;Lu CP;Liu YJ
通讯作者：
Liu YJ
影响因子：
3.1
作者：
Moral, CH;Del Castillo, EF;Carrasco, GN
通讯作者：
Carrasco, GN
影响因子：
--
作者：
Jacobs, Mary Ellen;DeSouza, Leroi V.;Klobutcher, Lawrence A.
通讯作者：
Klobutcher, Lawrence A.
影响因子：
3.8
作者：
Danelishvili, Lia;Stang, Bernadette;Bermudez, Luiz E.
通讯作者：
Bermudez, Luiz E.
使用原生动物嗜热四膜虫开发嗜水气单胞菌感染模型
影响因子：
2.1
作者：
Li, Jing;Zhang, Xiao-Lu;Lu, Cheng-Ping
通讯作者：
Lu, Cheng-Ping