Identification of Mycobacterium avium genes expressed during in vivo infection and the role of the oligopeptide transporter OppA in virulence.
Identification of Mycobacterium avium genes expressed during in vivo infection and the role of the oligopeptide transporter OppA in virulence.
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DOI：
10.1016/j.micpath.2014.09.010
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发表时间：
2014-11
影响因子：
3.8
通讯作者：
Bermudez, Luiz E.
中科院分区：
文献类型：
--
作者：
Danelishvili, Lia;Stang, Bernadette;Bermudez, Luiz E.
鸟支原体在艾滋病患者和其他免疫抑制疾病患者中引起播散性疾病，在慢性肺病患者中引起肺部感染。关于鸟支原体的发病机制还有很多需要了解的。利用播散性鸟分枝杆菌病小鼠模型，应用体内表达技术系统，鉴定了感染早期小鼠不同器官中上调的鸟分枝杆菌基因。鸟分枝杆菌oppA基因参与寡肽跨细胞膜的主动转运，在小鼠的肺、肝和脾中高表达。oppA基因转运结构域的突变导致巨噬细胞和小鼠的细菌衰减。通过蛋白相互作用实验，确定了两个假设的小蛋白MAV_2941 （73aa）和MAV_4320 （45aa）与OppA相互作用。mav_2941被证明是由细菌分泌到巨噬细胞细胞质中。MAV_2941的突变与巨噬细胞生长的显著损伤相关。有必要了解MAV_2941和MAV_4320功能中涉及的机制。
M. avium causes disseminated disease in patients with AIDS and other immunosuppressive conditions and pulmonary infections in individuals with chronic lung diseases. Much still need to be learn about the mechanisms of M. avium pathogenesis. Using a mouse model of disseminated M. avium disease, we applied an in vivo expression technology system and identified M. avium genes up-regulated in different organs of mice during early stage of infection. The M. avium oppA gene, involved in an active transport of oligopeptides across the cell membrane, was found highly expressed in lung, liver and spleen of mice. Mutation in the transport domain of the oppA gene resulted in bacterial attenuation in both macrophages and in mice. Using protein-protein interaction assay, it was determined that two hypothetical small proteins, MAV_2941 (73aa) and MAV_4320 (45aa), interact with OppA. MAV_2941was shown to be secreted by the bacterium into the macrophage cytoplasm. Mutations in MAV_2941 was associated with significant impairment of growth in macrophages. Understanding the mechanisms involved in the functions of MAV_2941 and MAV_4320 is warranted.
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影响因子：
4.8
作者：
Alberto Flores-Valdez, Mario;Morris, Rowan P.;Schoolnik, Gary K.
通讯作者：
Schoolnik, Gary K.
影响因子：
3.1
作者：
Lai, YC;Peng, HL;Chang, HY
通讯作者：
Chang, HY
影响因子：
2.6
作者：
Claverys, JP;Grossiord, B;Alloing, G
通讯作者：
Alloing, G
DOI：
10.1073/pnas.0610746104
发表时间：
2007-06-26
影响因子：
11.1
作者：
Danelishvili, Lia;Wu, Martin;Bermudez, Luiz E.
通讯作者：
Bermudez, Luiz E.
影响因子：
2.1
作者：
Danelishvili, L;Poort, MJ;Bermudez, LE
通讯作者：
Bermudez, LE