Silencing an Anopheles gambiae catalase and sulfhydryl oxidase increases mosquito mortality after a blood meal.
Silencing an Anopheles gambiae catalase and sulfhydryl oxidase increases mosquito mortality after a blood meal.
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DOI：
10.1002/arch.20238
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发表时间：
2008-07
影响因子：
2.2
通讯作者：
Foy, B. D.
中科院分区：
文献类型：
--
作者：
Magalhaes, T.;Brackney, D. E.;Beier, J. C.;Foy, B. D.
过氧化氢酶是一种有效的抗氧化剂，可能参与蚊子的血餐后体内平衡。这种酶分解H2 O2，防止羟基自由基（HO·）的形成。Quiescin是一种新分类的巯基氧化酶，其N-末端具有硫氧还蛋白基序，C-末端具有ERV 1样部分。这些蛋白质在细胞内或细胞外环境中产生二硫化物中起主要作用，因此参与氧化还原反应。在寻找分子作为新的灭蚊策略的目标，我们已经沉默了过氧化氢酶和一个假定的quiescin/巯基氧化酶（QSOX），从非洲疟疾载体冈比亚按蚊，通过RNA干扰（RNAi）实验。我们观察到过氧化氢酶和QSOX沉默昆虫的存活率在血液消化后比对照降低，这很可能是由于蚊子抑制和/或预防由血餐衍生的氧化应激引起的损伤的能力受损。在过氧化氢酶沉默的蚊子中，过氧化氢酶活性降低到低水平，更高的死亡率效应更加突出。脂质过氧化较高的QSOX沉默的蚊子，这表明这种蛋白质的参与氧化还原稳态后的血餐。这项研究指出了参与抗氧化反应和氧化还原代谢的分子作为新型灭蚊策略靶点的潜力，并提供了一种寻找可靶向蚊子分子的筛选方法。
Catalase is a potent antioxidant, likely involved in post-blood meal homeostasis in mosquitoes. This enzyme breaks down H2O2, preventing the formation of the hydroxyl radical (HO•). Quiescins are newly classified sulfhydryl oxidases that bear a thioredoxin motif at the N-terminal and an ERV1-like portion at the C-terminal. These proteins have a major role in generating disulfides in intra- or extracellular environments, and thus participate in redox reactions. In the search for molecules to serve as targets for novel anti-mosquito strategies, we have silenced a catalase and a putative quiescin/sulfhydryl oxidase (QSOX), from the African malaria vector Anopheles gambiae, through RNA interference (RNAi) experiments. We observed that the survival of catalase- and QSOX-silenced insects was reduced over controls following blood digestion, most likely due to the compromised ability of mosquitoes to scavenge and/or prevent damage caused by blood meal-derived oxidative stress. The higher mortality effect was more accentuated in catalase-silenced mosquitoes, where catalase activity was reduced to low levels. Lipid peroxidation was higher in QSOX-silenced mosquitoes suggesting the involvement of this protein in redox homeostasis following a blood meal. This study points to the potential of molecules involved in antioxidant response and redox metabolism to serve as targets of novel anti-mosquito strategies and offers a screening methodology for finding targetable mosquito molecules.
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影响因子：
2.2
作者：
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通讯作者：
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