Early neural crest induction requires an initial inhibition of Wnt signals.
Early neural crest induction requires an initial inhibition of Wnt signals.
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DOI：
10.1016/j.ydbio.2012.02.029
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发表时间：
2012-05-01
影响因子：
2.7
通讯作者：
Mayor R
中科院分区：
文献类型：
--
作者：
Steventon B;Mayor R
神经嵴（NC）的诱导是一个漫长的过程，持续通过原肠胚和神经胚阶段。为了揭示NC诱导的其他阶段，我们进行了一系列外植体，其中将不同的已知诱导组织与预期NC一起沿着。有趣的是，背外侧边缘区（DLMZ）仅能够促进神经板边界（NPB）标记物的子集的表达，而不存在特异性NC标记物。然后，我们分析了NPB基因Hairy 2a和Dlx 5的BMP和Wnt信号的时间要求，与神经板（NP）和NC基因的表达相比。虽然NP在早期原肠胚阶段对BMP水平敏感，但Hairy 2a/Dlx 5表达不受影响。后来，当NC标记物需要抑制时，NP在原肠胚形成晚期对BMP水平变得不敏感。NP需要在原肠胚形成之前抑制Wnt信号，但在早期原肠胚阶段期间当Hairy 2a/Dlx 5需要抑制Wnt信号时变得不敏感。Wnt信号传导的增加对于在原肠胚晚期从NPB到NC的转换是重要的。除了揭示一个额外的不同的信号事件在NC诱导，这项工作强调整合的重要性，在复杂的组织，如脊椎动物外胚层的图案化的时间和水平的信号活动。神经板边界是NC感应过程中的一个独特步骤。神经板和边界标记物在不同时间对Wnt和BMP信号作出反应。在原肠胚形成期间，神经板边界需要抑制Wnt信号。Wnt的增加使更特异的神经板边界标记物的出现成为可能。
Neural crest (NC) induction is a long process that continues through gastrula and neurula stages. In order to reveal additional stages of NC induction we performed a series of explants where different known inducing tissues were taken along with the prospective NC. Interestingly the dorso-lateral marginal zone (DLMZ) is only able to promote the expression of a subset of neural plate border (NPB) makers without the presence of specific NC markers. We then analysed the temporal requirement for BMP and Wnt signals for the NPB genes Hairy2a and Dlx5, compared to the expression of neural plate (NP) and NC genes. Although the NP is sensitive to BMP levels at early gastrula stages, Hairy2a/Dlx5 expression is unaffected. Later, the NP becomes insensitive to BMP levels at late gastrulation when NC markers require an inhibition. The NP requires an inhibition of Wnt signals prior to gastrulation, but becomes insensitive during early gastrula stages when Hairy2a/Dlx5 requires an inhibition of Wnt signalling. An increase in Wnt signalling is then important for the switch from NPB to NC at late gastrula stages. In addition to revealing an additional distinct signalling event in NC induction, this work emphasizes the importance of integrating both timing and levels of signalling activity during the patterning of complex tissues such as the vertebrate ectoderm. ► The neural plate border is a distinct step in the NC induction process. ► Neural plate and border markers respond to Wnt and BMP signals at different times. ► The neural plate border requires an inhibition of Wnt signals during gastrulation. ► An increase in Wnt enables the onset of more specific neural plate border markers.
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影响因子：
64.8
作者：
Glinka, A;Wu, W;Niehrs, C
通讯作者：
Niehrs, C
影响因子：
64.8
作者：
Bouwmeester, T;Kim, SH;DeRobertis, EM
通讯作者：
DeRobertis, EM
影响因子：
4.6
作者：
Aybar, MJ;Nieto, MA;Mayor, R
通讯作者：
Mayor, R
影响因子：
10.5
作者：
CHRISTIAN, JL;MOON, RT
通讯作者：
MOON, RT
DOI：
10.1073/pnas.1010740107
发表时间：
2011-01-04
影响因子：
11.1
作者：
de Croze, Noemie;Maczkowiak, Frederique;Monsoro-Burq, Anne H.
通讯作者：
Monsoro-Burq, Anne H.