Three-Dimensional Analysis of Peeled Internal Limiting Membrane Using Focused Ion Beam/Scanning Electron Microscopy.
Three-Dimensional Analysis of Peeled Internal Limiting Membrane Using Focused Ion Beam/Scanning Electron Microscopy.
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DOI：
10.1167/tvst.7.1.15
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发表时间：
2018-03
影响因子：
3
通讯作者：
Nakamura KI
中科院分区：
文献类型：
--
作者：
Hirata A;Murata K;Hayashi K;Nakamura KI
为探讨玻璃体切割术中内界膜剥离对Müller细胞损伤的影响，采用聚焦离子束/扫描电镜（FIB/SEM）观察玻璃体切割术中内界膜的超微结构。对黄斑裂孔组和特发性视网膜前膜组共12例内界膜进行FIB/SEM观察。分析了内界膜的三维结构。黄斑裂孔组每单位面积内界膜（100 μm2）细胞碎片数为5.07 ± 1.03个。细胞碎片的总体积为3.54 ± 1.24 μm3/100 μm2。相比之下，视网膜前膜组中的细胞碎片数量为12.85 ± 3.45/100 μm2，细胞碎片的总体积为10.45 ± 2.77 μm3/100 μm2。两个数值的数据均显著高于黄斑裂孔组中观察到的数值（分别为P = 0.0024和P = 0.0022，Mann-Whitney U检验）。两组间细胞碎片的平均体积无统计学差异。本研究中检查的所有内界膜均显示内界膜视网膜表面有细胞碎片。与黄斑裂孔相比，视网膜前膜显示出更高的细胞碎片数量和总体积，表明视网膜前膜的内界膜剥离可能具有更高的引起视网膜内层损伤的风险。FIB/SEM是对内界膜进行三维定量分析的有效工具。
To reevaluate the effect of internal limiting membrane peeling during vitrectomy on the Müller cell damage, we examined the ultrastructure of the internal limiting membrane by using focused ion beam/scanning electron microscopy (FIB/SEM). A total of 12 internal limiting membranes obtained during surgery in both the macular hole and the idiopathic epiretinal membrane groups were processed for observation by FIB/SEM. Three-dimensional structures of the internal limiting membrane were analyzed. The number of cell fragments in the macular hole group was 5.07 ± 1.03 per unit area of internal limiting membrane (100 μm2). The total volume of cell fragments was 3.54 ± 1.24 μm3/100 μm2. In contrast, the number of cell fragments in the epiretinal membrane group was 12.85 ± 3.45/100 μm2, and the total volume of cell fragments was 10.45 ± 2.77 μm3/100 μm2. Data for both values were significantly higher than those observed in the macular hole group (P = 0.0024 and P = 0.0022, respectively, Mann-Whitney U test). No statistical difference was found for the mean volume of the cell fragment between the two groups. All of the internal limiting membrane examined in this study showed cell fragments on the retinal surface of the internal limiting membrane. As compared with macular hole, epiretinal membrane exhibited a higher number and total volume of cell fragments, indicating that internal limiting membrane peeling for epiretinal membrane might have a higher risk of causing inner retinal damage. FIB/SEM was a useful tool for three-dimensional quantitative analysis of the internal limiting membrane.
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影响因子：
2.2
作者：
Schmidt, Franziska;Kuehbacher, Markus;Zehbe, Rolf
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
3.3
作者：
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通讯作者：
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DOI：
10.1097/iae.0000000000001263
发表时间：
2017-05-01
影响因子：
3.3
作者：
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通讯作者：
Thompson, John T.