Three-dimensional ultrastructural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography.
Three-dimensional ultrastructural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography.
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DOI：
10.1038/srep39435
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发表时间：
2016-12-20
影响因子：
4.6
通讯作者：
Nakamura KI
中科院分区：
文献类型：
--
作者：
Hirashima S;Ohta K;Kanazawa T;Okayama S;Togo A;Uchimura N;Kusukawa J;Nakamura KI
对正常组织的准确理解为分析疾病和再生过程等异常提供了必要的数据。此外，了解靶组织的适当结构及其微环境可能有助于成功的新治疗策略。许多研究对牙周韧带的性质和结构进行了研究，但对正常牙周韧带细胞的三维结构仍知之甚少。在这项研究中，我们使用聚焦离子束/扫描电子显微镜断层扫描技术研究了PDL细胞的整体三维超微结构，并对其结构特性进行了定量分析，确定了它们在胶原纤维方向上的取向。牙周膜细胞相互接触，在牙骨质和牙槽骨之间形成广泛的网状网。水平纤维区的细胞体积明显大于其他区，而各向异性低于其他区。此外，各区域内细胞向PDL纤维的方向与PDL纤维不平行。由于类似的评估被认为使用传统的二维方法具有挑战性，这些新的3D发现可能有助于全面理解和分析PDL。
The accurate comprehension of normal tissue provides essential data to analyse abnormalities such as disease and regenerative processes. In addition, understanding the proper structure of the target tissue and its microenvironment may facilitate successful novel treatment strategies. Many studies have examined the nature and structure of periodontal ligaments (PDLs); however, the three-dimensional (3D) structure of cells in normal PDLs remains poorly understood. In this study, we used focused ion beam/scanning electron microscope tomography to investigate the whole 3D ultrastructure of PDL cells along with quantitatively analysing their structural properties and ascertaining their orientation to the direction of the collagen fibre. PDL cells were shown to be in contact with each other, forming a widespread mesh-like network between the cementum and the alveolar bone. The volume of the cells in the horizontal fibre area was significantly larger than in other areas, whereas the anisotropy of these cells was lower than in other areas. Furthermore, the orientation of cells to the PDL fibres was not parallel to the PDL fibres in each area. As similar evaluations are recognized as being challenging using conventional two-dimensional methods, these novel 3D findings may contribute necessary knowledge for the comprehensive understanding and analysis of PDLs.
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通过块面扫描电子显微镜揭示的足细胞的三维结构。
影响因子：
4.6
作者：
Ichimura K;Miyazaki N;Sadayama S;Murata K;Koike M;Nakamura K;Ohta K;Sakai T
通讯作者：
Sakai T
影响因子：
3.4
作者：
Nichol JW;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
4.4
作者：
Haniffa, Muzlifah A.;Wang, Xiao-Nong;Collin, Matthew P.
通讯作者：
Collin, Matthew P.
影响因子：
3.5
作者：
BEERTSEN, W;EVERTS, V
通讯作者：
EVERTS, V
影响因子：
4.6
作者：
Hirashima S;Ohta K;Kanazawa T;Uemura K;Togo A;Yoshitomi M;Okayama S;Kusukawa J;Nakamura K
通讯作者：
Nakamura K