Modulation of Pseudomonas aeruginosa surface-associated group behaviors by individual amino acids through c-di-GMP signaling.
Modulation of Pseudomonas aeruginosa surface-associated group behaviors by individual amino acids through c-di-GMP signaling.
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DOI：
10.1016/j.resmic.2011.04.014
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发表时间：
2011-09
影响因子：
2.6
通讯作者：
O'Toole, George A.
中科院分区：
文献类型：
--
作者：
Bernier, Steve P.;Ha, Dae-Gon;Khan, Wajiha;Merritt, Judith H.;O'Toole, George A.
为了在囊性纤维化肺中定居，铜绿假单胞菌建立了称为生物膜的固着群落。尽管信号分子c-di-GMP控制从能动生长到固着生长的转变，但调节生物膜形成所需的环境信号仍不清楚。使用先前在囊性纤维化痰中鉴定的19种氨基酸的相关体内浓度，我们证明精氨酸、鸟氨酸、异亮氨酸、亮氨酸、缬氨酸、苯丙氨酸和酪氨酸在体外强烈促进生物膜形成。在七种促进生物膜的氨基酸中，只有精氨酸也完全抑制了铜绿假单胞菌在半固体表面上聚集的能力，这表明精氨酸可能是有利于固着生活方式的环境线索。突变两个记录的生物膜形成所需的二鸟苷酸环化酶（SadC和RoeA）减少生物膜的形成和恢复群集运动在含精氨酸的培养基。精氨酸生长增加了细胞内的c-di-GMP水平，这种增加依赖于SadC和RoeA二鸟苷酸环化酶。菌株突变的sadC，roeA或两者也显示出减少生物膜形成时，生长与其他生物膜促进氨基酸。总之，这些结果表明，氨基酸可以调节生物膜形成和群集运动，至少在部分，通过控制细胞内水平的c-di-GMP。
To colonize the cystic fibrosis lung, Pseudomonas aeruginosa establishes sessile communities referred to as biofilms. Although the signaling molecule c-di-GMP governs the transition from motile to sessile growth, the environmental signal(s) required to modulate biofilm formation remain unclear. Using relevant in vivo concentrations of the 19 amino acids previously identified in cystic fibrosis sputum, we demonstrated that arginine, ornithine, isoleucine, leucine, valine, phenylalanine and tyrosine robustly promoted biofilm formation in vitro. Among the seven biofilm-promoting amino acids, only arginine also completely repressed the ability of P. aeruginosa to swarm over semi-solid surfaces, suggesting that arginine may be an environmental cue favoring a sessile lifestyle. Mutating two documented diguanylate cyclases required for biofilm formation (SadC and RoeA) reduced biofilm formation and restored swarming motility on arginine-containing medium. Growth on arginine increased the intracellular levels of c-di-GMP, and this increase was dependent on the SadC and RoeA diguanylate cyclases. Strains mutated in sadC, roeA or both also showed a reduction in biofilm formation when grown with the other biofilm-promoting amino acids. Taken together, these results suggest that amino acids can modulate biofilm formation and swarming motility, at least in part, by controlling the intracellular levels of c-di-GMP.
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影响因子：
3.2
作者：
Eschbach, M;Schreiber, K;Schobert, M
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3.2
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