Relationship among serum levels of IL-6, sIL-6R, s gp130 and CD126 on T-cell in HIV-1 infected and uninfected men participating in the Los Angeles Multi-Center AIDS Cohort Study.
Relationship among serum levels of IL-6, sIL-6R, s gp130 and CD126 on T-cell in HIV-1 infected and uninfected men participating in the Los Angeles Multi-Center AIDS Cohort Study.
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DOI：
10.1371/journal.pone.0290702
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
白细胞介素6（IL-6）通过其独特的IL-6受体（IL-6 R）亚基和白细胞介素6信号转导β亚基糖蛋白130（gp 130）的异二聚体信号复合物激活细胞。本研究的目的是探讨HIV-1感染者和未感染者血清IL-6、sIL-6 R、sgp 130水平与T细胞IL-6受体α亚单位（CD 126）相对荧光强度（RFI）之间的关系。从69名接受高效抗逆转录病毒治疗（HAART）的HIV-1感染男性（平均年龄49.1岁）和52名未感染HIV-1的男性（平均年龄54.3岁）中获得血液样本。所有男性都参加了洛杉矶多中心艾滋病队列研究（MACS）。采用酶联免疫法和T细胞表型分析法检测血清IL-6、sIL-6 R、sgp 130水平，流式细胞术检测CD 4+、CD 8+细胞表面CD 126的RFI。HIV-1感染者血清IL-6、sIL-6 R、sgp 130和CD 4+上的CD 126 RFI的平均水平分别为4.34 pg/mL、39.3 ng/mL、349 ng/mL和526 RFI; HIV-1未感染者血清IL-6、sIL-6 R、sgp 130和CD 126 RFI的平均水平分别为2.74 pg/mL、41.9 ng/mL、318 ng/mL和561 RFI。HIV-1感染者与未感染者血清IL-6、sIL-6 R水平无显著性差异（P>0.05）。血浆HIV-1 RNA与IL-6（p<0.001）、sIL-6 R（p = 0.002）水平呈正相关，与sgp 130无相关性（p = 0.339）。此外，在HIV-1感染者中，血清IL-6水平与CD 4+上CD 126的RFI呈负相关（p = 0.037），血清sgp 130水平与sIL-6 R呈正相关（p = 0.021）。了解生物学变异，健康个体和患病个体之间生物标志物血液水平的差异，对于选择疾病阶段和进展的适当测试非常重要。我们的数据表明，在HIV-1感染的治疗阶段，IL-6和sIL-R6之间没有相关性。IL-6及其受体的作用和血液水平在疾病进展的每个阶段可能不同。
Interleukin 6 (IL-6) activates cells through its unique heterodimeric signaling complex of IL-6 receptor (IL6R) subunit and interleukin 6 signal transducer β-subunit glycoprotein 130 (gp130). The objective of this study was to investigate associations among serum levels of IL-6, sIL-6R, sgp130 and relative fluorescence intensity (RFI) of the α-subunit of the IL-6 receptor (CD126) on T-cells of HIV-1 infected and uninfected men. Blood samples were obtained from 69 HIV-1-infected men on Highly Active Antiretroviral Therapy (HAART) with mean age of 49.1 and 52 HIV-1-uninfected with mean age of 54.3 years -. All men were participating in the Los Angeles Multi-Center AIDS Cohort Study (MACS). Serum levels of IL-6, sIL-6R, sgp130 were measured by enzyme-linked immunoassays and T-cell phenotypic analysis and RFI of CD126 on CD4+ and CD8+ by flow cytometry. Mean serum levels of IL-6, sIL6R, sgp130 and of CD126 RFI on CD4+ were 4.34 pg/mL, 39.3 ng/mL, 349 ng/mL and 526 RFI respectively for HIV-1-infected men and 2.74 pg/mL, 41.9 ng/mL, 318 ng/mL and 561 RFI respectively for HIV-1-uninfected men. The mean serum concentrations of IL-6, sIL-6R in HIV-1-infected and uninfected men were not significantly different (p>0.05). There was a positive correlation between plasma HIV-1 RNA and the levels of IL-6 (p<0.001), sIL6R (p = 0.002) but no correlation with sgp130 (p = 0.339). In addition, there was a negative correlation between serum levels of IL-6 with RFI of CD126 on CD4+ (p = 0.037) and a positive correlation between serum levels of sgp130 (p = 0.021) and sIL-6R in HIV-1-infected men. Knowledge of biological variation, differences in the blood levels of biomarkers among healthy individuals and individuals experiencing illness, are very important for selection of appropriate tests for stage and progression of disease. Our data suggest no correlation among IL-6, and sIL-R6, in the treated phase of HIV-1 infection. The action and blood level of IL-6 and its receptors may be different at each stage of a disease progression.
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DOI：
10.1038/s41577-023-00856-y
发表时间：
2023-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
158.5
作者：
FAHEY, JL;TAYLOR, JMG;GIORGI, JV
通讯作者：
GIORGI, JV
DOI：
10.1002/cyto.990090605
发表时间：
1988-11-01
期刊：
CYTOMETRY
影响因子：
--
作者：
SCHMID, I;SCHMID, P;GIORGI, JV
通讯作者：
GIORGI, JV
影响因子：
15.8
作者：
Kuller LH;Tracy R;Belloso W;De Wit S;Drummond F;Lane HC;Ledergerber B;Lundgren J;Neuhaus J;Nixon D;Paton NI;Neaton JD;INSIGHT SMART Study Group
通讯作者：
INSIGHT SMART Study Group
影响因子：
64.5
作者：
HIBI, M;MURAKAMI, M;KISHIMOTO, T
通讯作者：
KISHIMOTO, T