Bioaerosols from a food waste composting plant affect human airway epithelial cell remodeling genes.
Bioaerosols from a food waste composting plant affect human airway epithelial cell remodeling genes.
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DOI：
10.3390/ijerph110100337
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发表时间：
2013-12-24
影响因子：
--
通讯作者：
Chuang CY
中科院分区：
文献类型：
--
作者：
Chang MW;Lee CR;Hung HF;Teng KS;Huang H;Chuang CY
食物垃圾处理厂的堆肥过程会产生空气中的生物气溶胶，有可能损害人类气道上皮细胞。持续的炎症和修复反应诱导气道重塑和呼吸系统损伤。本研究旨在探讨堆肥厂生物气溶胶对人肺粘液表皮样细胞NCI-H292气道重塑相关基因表达的影响。不同类型的微生物在堆肥厂检测，使用琼脂培养法。实时定量聚合酶链反应被用来定量烟曲霉的水平和重塑基因的概况。实时荧光定量PCR结果表明，A.堆肥室内烟曲霉孢子数小于102个。使用鲎变形细胞溶解物试验测定现场生物气溶胶中的内毒素。内毒素水平取决于颗粒物（PM）的类型，粗颗粒（2.5-10 μm）的内毒素水平高于细颗粒（0.5-2.5 μm）。在暴露于现场生物气溶胶样品的条件培养基后，NCI-H292细胞显示促炎性白细胞介素（IL）-6释放增加，表皮生长因子受体（EGFR）、转化生长因子（TGF）-β1和细胞周期蛋白依赖性激酶抑制剂1（p21 WAF 1/CIP 1）基因表达活化，但基质金属肽酶（MMP）-9基因表达不活化。空气中的内毒素水平较高的堆肥大厅内比他们在其他地区，他们与PM。这表明，堆肥厂空气中的生物气溶胶中除了A.烟曲霉毒素引起NCI-H292细胞中炎性细胞因子分泌并增加重塑基因的表达。因此，有必要监测食品堆肥厂生物气溶胶中可能影响工人健康的潜在危险物质。
The composting procedure in food waste plants generates airborne bioaerosols that have the potential to damage human airway epithelial cells. Persistent inflammation and repair responses induce airway remodeling and damage to the respiratory system. This study elucidated the expression changes of airway remodeling genes in human lung mucoepidermoid NCI-H292 cells exposed to bioaerosols from a composting plant. Different types of microorganisms were detectable in the composting plant, using the agar culture method. Real-time polymerase chain reaction was used to quantify the level of Aspergillus fumigatus and the profile of remodeling genes. The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 102 conidia. The endotoxins in the field bioaerosols were determined using a limulus amebocyte lysate test. The endotoxin levels depended on the type of particulate matter (PM), with coarse particles (2.5–10 μm) having higher endotoxin levels than did fine particles (0.5–2.5 μm). After exposure to the conditioned medium of field bioaerosol samples, NCI-H292 cells showed increased pro-inflammatory interleukin (IL)-6 release and activated epidermal growth factor receptor (EGFR), transforming growth factor (TGF)-β1 and cyclin-dependent kinase inhibitor 1 (p21WAF1/CIP1) gene expression, but not of matrix metallopeptidase (MMP)-9. Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM. This suggested that airborne bioaerosols in the composting plant contained endotoxins and microorganisms besides A. fumigatus that cause the inflammatory cytokine secretion and augment the expression of remodeling genes in NCI-H292 cells. It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.
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DOI：
10.1078/s1438-4639(04)70014-0
发表时间：
2000-10-01
影响因子：
6
作者：
Fischer, G;Müller, T;Dott, W
通讯作者：
Dott, W
DOI：
10.1152/ajplung.00466.2001
发表时间：
2003-06-01
影响因子：
4.9
作者：
Carnevali, S;Petruzzelli, S;Giuntini, C
通讯作者：
Giuntini, C
影响因子：
2
作者：
Gilbert, Yan;Veillette, Marc;Duchaine, Caroline
通讯作者：
Duchaine, Caroline
影响因子：
8.9
作者：
Adhikari, A;Reponen, T;LeMasters, G
通讯作者：
LeMasters, G
影响因子：
4.8
作者：
Kim, Sangmin;Choi, Jae Hyuck;Yang, Jung-Hyun
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Yang, Jung-Hyun