Nup154, a new Drosophila gene essential for male and female gametogenesis is related to the nup155 vertebrate nucleoporin gene.
Nup154, a new Drosophila gene essential for male and female gametogenesis is related to the nup155 vertebrate nucleoporin gene.
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DOI：
10.1083/jcb.142.5.1195
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发表时间：
1998-09-07
期刊：
影响因子：
--
通讯作者：
Malva C
中科院分区：
文献类型：
--
作者：
Gigliotti S;Callaini G;Andone S;Riparbelli MG;Pernas-Alonso R;Hoffmann G;Graziani F;Malva C
果蝇的Nup154基因编码的蛋白质与已知的核孔蛋白（大鼠Nup155和酵母Nup170和Nup157）相似。Nup154的亚型突变等位基因影响雌性和雄性的生育力，允许调查在卵子发生和精子发生的各个步骤中的基因功能。Nup154在睾丸囊肿形成、精母细胞增殖和减数分裂进程的控制中是必需的。在卵巢中，Nup154是卵室发育和卵母细胞生长所必需的。在雄性和雌性生殖系以及其他几种细胞类型中，Nup154蛋白在核膜上检测到，但也存在于细胞核内。核内定位以前没有被描述为大鼠Nup155或酵母Nup170和Nup157。在突变的卵室中，Nup154蛋白积累在细胞质中，而在核膜中几乎检测不到。用mAb414抗体检测到的含有核孔蛋白的FG重复在Nup154突变等位基因中也有一定程度的错误定位。这表明，Nup154可能需要定位核孔复合物内的其他核孔蛋白，如先前所证明的酵母Nup170。另一方面，核纤层蛋白定位没有明显的缺陷，表明Nup155突变不影响核膜的整体完整性。然而，超微结构分析表明，在突变细胞的核被膜的形态改变核孔复合物附近。最后，在Nup154突变等位基因中观察到的表型的多样性表明该基因在细胞生理学中起着至关重要的作用。
The Nup154 gene of Drosophila encodes a protein showing similarity with known nucleoporins: rat Nup155 and yeast Nup170 and Nup157. Hypomorphic mutant alleles of Nup154 affected female and male fertility, allowing investigation of the gene function in various steps of oogenesis and spermatogenesis. Nup154 was required in testes for cyst formation, control of spermatocyte proliferation and meiotic progression. In ovaries, Nup154 was essential for egg chamber development and oocyte growth. In both the male and female germ line, as well as in several other cell types, the Nup154 protein was detected at the nuclear membrane, but was also present inside the nucleus. Intranuclear localization has not previously been described for rat Nup155 or yeast Nup170 and Nup157. In mutant egg chambers the Nup154 protein accumulated in the cytoplasm, while it was only barely detected at the nuclear envelopes. FG repeats containing nucleoporins detected with mAb414 antibody were also mislocalized to a certain extent in Nup154 mutant alleles. This suggests that Nup154 could be required for localizing other nucleoporins within the nuclear pore complex, as previously demonstrated for the yeast Nup170. On the other hand, no evident defects in lamin localization were observed, indicating that Nup155 mutations did not affect the overall integrity of the nuclear envelope. However, ultrastructural analyses revealed that in mutant cells the morphology of the nuclear envelope was altered near the nuclear pore complexes. Finally, the multiplicity of phenotypes observed in Nup154 mutant alleles suggests that this gene plays a crucial role in cell physiology.
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影响因子：
30.8
作者：
GISH, W;STATES, DJ
通讯作者：
STATES, DJ
影响因子：
10.5
作者：
JONGENS, TA;ACKERMAN, LD;JAN, YN
通讯作者：
JAN, YN
DOI：
10.1083/jcb.131.5.1133
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Aitchison JD;Rout MP;Marelli M;Blobel G;Wozniak RW
通讯作者：
Wozniak RW
影响因子：
2.7
作者：
GIGLIOTTI, S;CAVALIERE, V;MALVA, C
通讯作者：
MALVA, C
影响因子：
7.8
作者：
Aitchison, JD;Blobel, G;Rout, MP
通讯作者：
Rout, MP