Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin.
Comparative transcriptome analysis reveals significant metabolic alterations in eri-silkworm (Samia cynthia ricini) haemolymph in response to 1-deoxynojirimycin.
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DOI：
10.1371/journal.pone.0191080
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Xu JP
中科院分区：
文献类型：
--
作者：
Zhang SZ;Yu HZ;Deng MJ;Ma Y;Fei DQ;Wang J;Li Z;Meng Y;Xu JP
蓖麻蚕（Samia cynthia ricini）（鳞翅目：蚕蛾科）是一种重要的商业产丝昆虫;然而，与蚕相反，桑葚对这种昆虫有毒，因为叶子含有1-脱氧野尻霉素（DNJ）成分。本研究通过对蓖麻蚕血淋巴的转录组学分析，探讨蓖麻蚕血淋巴对DNJ反应的分子机制。结果表明，与对照组相比，2%DNJ组12 h后表达上调的基因有577个，表达下调的基因有288个。基于功能分析的结果，这些DEG与核糖体、糖酵解、N-聚糖生物合成和氧化磷酸化相关。特别地，根据KEGG分析，138个DEG参与能量代谢、糖代谢和脂质代谢，并且通过逆转录定量PCR（RT-qPCR）证实了9个DEG的表达变化。因此，DNJ诱导蓖麻蚕血淋巴中的显著代谢改变。本研究结果将为DNJ对蓖麻蚕的毒性作用研究奠定基础，并为人类新药的开发提供参考。
Samia cynthia ricini (Lepidoptera: Saturniidae) is an important commercial silk-producing insect; however, in contrast to the silkworm, mulberry leaves are toxic to this insect because the leaves contain the component 1-deoxynojirimycin (DNJ). A transcriptomic analysis of eri-silkworm haemolymph was conducted to examine the genes related to different metabolic pathways and to elucidate the molecular mechanism underlying eri-silkworm haemolymph responses to DNJ. Eight hundred sixty-five differentially expressed genes (DEGs) were identified, among which 577 DEGs were up-regulated and 288 DEGs were down-regulated in the 2% DNJ group compared to control (ddH2O) after 12h. Based on the results of the functional analysis, these DEGs were associated with ribosomes, glycolysis, N-glycan biosynthesis, and oxidative phosphorylation. In particular, according to the KEGG analysis, 138 DEGs were involved in energy metabolism, glycometabolism and lipid metabolism, and the changes in the expression of nine DEGs were confirmed by reverse transcription quantitative PCR (RT-qPCR). Thus, DNJ induced significant metabolic alterations in eri-silkworm haemolymph. These results will lay the foundation for research into the toxic effects of DNJ on eri-silkworm as a model and provide a reference for the exploitation of new drugs in humans.
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影响因子：
3.7
作者：
Deng MJ;Lin XD;Wen CW;Dong MJ;Lin QT;Zhang SZ;Xu JP
通讯作者：
Xu JP
影响因子：
3.8
作者：
Ahn, Seung-Joon;Vogel, Heiko;Heckel, David G.
通讯作者：
Heckel, David G.
DOI：
10.1111/j.1432-1033.1984.tb08253.x
发表时间：
1984-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
HETTKAMP, H;LEGLER, G;BAUSE, E
通讯作者：
BAUSE, E
影响因子：
9.7
作者：
Guerrera, M;Ladisch, S
通讯作者：
Ladisch, S
影响因子：
6.8
作者：
Nath, BS;Suresh, A;Kumar, RPS
通讯作者：
Kumar, RPS