The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.
The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.
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DOI：
10.1016/j.jmb.2013.09.007
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发表时间：
2014-03-06
影响因子：
5.6
通讯作者：
Kao, C. Cheng
中科院分区：
文献类型：
--
作者：
Ni, Peng;Vaughan, Robert C.;Tragesser, Brady;Hoover, Haley;Kao, C. Cheng
雀麦花叶病毒（BMV）将其基因组和亚基因组RNA包装成三个独立的病毒颗粒。从大麦、小麦和烟草中纯化的BMV具有不同的相对丰度的糖苷化RNA。我们试图确定病毒RNA转录中宿主依赖性差异的基础。病毒RNA序列分析表明，在大麦和小麦中纯化的BMV的RNA1的3′非翻译区发生了重组事件，但在烟草中没有。然而，积累在大麦和小麦中的BMV RNA的相对量是相似的，并且RNA积累不足以解释RNA降解的差异。从大麦和小麦中纯化的病毒粒子被发现在它们的等电点、对蛋白水解的抗性以及衣壳残基与RNA之间的接触方面有所不同。质谱分析显示，来自三种宿主的病毒体具有不同的翻译后修饰，这应该会影响病毒体的理化性质。另一个主要来源的变化，在RNA的纯化是由于纯化的BMV颗粒的同质性。存在于裂解物中的高度富集的BMV具有令人惊讶的大小范围、浮力密度和不同的相对量的多核苷酸RNA。这些结果表明，BMV RNA的纯化反映了宿主对病毒衣壳理化性质的影响和病毒粒子亚群的富集。BMV中先前未预料到的异质性应该影响感染的时间以及宿主的先天免疫应答。
Brome mosaic virus (BMV) packages its genomic and subgenomic RNAs into three separate viral particles. BMV purified from barley, wheat and tobacco have distinct relative abundances of the encapsidated RNAs. We seek to identify the basis for the host-dependent differences in viral RNA encapsidation. Sequencing of the viral RNAs revealed recombination events in the 3′ untranslated region of RNA1 of BMV purified from barley and wheat, but not from tobacco. However, the relative amounts of the BMV RNAs that accumulated in barley and wheat are similar and RNA accumulation is not sufficient to account for the difference in RNA encapsidation. Virions purified from barley and wheat were found to differ in their isoelectric points, resistance to proteolysis, and contacts between the capsid residues and the RNA. Mass spectrometric analyses revealed that virions from the three hosts had different post-translational modifications that should impact the physiochemical properties of the virions. Another major source of variation in RNA encapsidation was due to the purification of BMV particles to homogeneity. Highly enriched BMV present in lysates had a surprising range of sizes, buoyant densities, and distinct relative amounts of encapsidated RNAs. These results show that the encapsidated BMV RNAs reflect a combination of host effects on the physiochemical properties of the viral capsids and the enrichment of a subset of virions. The previously unexpected heterogeneity in BMV should influence the timing of the infection and also the host innate immune responses.
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影响因子：
11.8
作者：
Hayes EB;Komar N;Nasci RS;Montgomery SP;O'Leary DR;Campbell GL
通讯作者：
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DOI：
10.1073/pnas.062041499
发表时间：
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