Noninvasive assessment of cardiac abnormalities in experimental autoimmune myocarditis by magnetic resonance microscopy imaging in the mouse.
Noninvasive assessment of cardiac abnormalities in experimental autoimmune myocarditis by magnetic resonance microscopy imaging in the mouse.
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DOI：
10.3791/51654
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发表时间：
2014-06-20
期刊：
影响因子：
--
通讯作者：
Reddy J
中科院分区：
文献类型：
--
作者：
Massilamany C;Khalilzad-Sharghi V;Gangaplara A;Steffen D;Othman SF;Reddy J
心肌炎是心肌的炎症，但只有约10%的受影响者表现出该疾病的临床表现。为了研究心肌损伤的免疫事件，各种小鼠心肌炎模型已被广泛使用。本研究涉及A/J小鼠心肌肌球蛋白重链（Myhc）-α 334-352诱导的实验性自身免疫性心肌炎（EAM）;受影响的动物发生淋巴细胞性心肌炎，但无明显临床体征。在该模型中，显示了磁共振显微镜（MRM）作为一种非侵入性方式用于确定Myhc-α 334-352免疫动物的心脏结构和功能变化的效用。使用9.4 T（400 MHz）89 mm垂直核心孔扫描仪对EAM和健康小鼠进行成像，该扫描仪配备有4 cm千足虫射频成像探头和100 G/cm三轴梯度。使用基于梯度回波的电影脉冲序列从麻醉动物中采集心脏图像，并通过呼吸和脉搏血氧饱和度监测动物。分析显示，与健康小鼠相比，EAM小鼠的心室壁厚度增加，心室内径相应减少。这些数据表明，炎症心脏的形态和功能变化可以通过活体动物的MRM进行无创监测。总之，MRM提供了一个优势，评估由感染性病原体引起的疾病的心肌损伤的进展和消退，以及对治疗的反应。
Myocarditis is an inflammation of the myocardium, but only ~10% of those affected show clinical manifestations of the disease. To study the immune events of myocardial injuries, various mouse models of myocarditis have been widely used. This study involved experimental autoimmune myocarditis (EAM) induced with cardiac myosin heavy chain (Myhc)-α 334–352 in A/J mice; the affected animals develop lymphocytic myocarditis but with no apparent clinical signs. In this model, the utility of magnetic resonance microscopy (MRM) as a non-invasive modality to determine the cardiac structural and functional changes in animals immunized with Myhc-α 334–352 is shown. EAM and healthy mice were imaged using a 9.4 T (400 MHz) 89 mm vertical core bore scanner equipped with a 4 cm millipede radio-frequency imaging probe and 100 G/cm triple axis gradients. Cardiac images were acquired from anesthetized animals using a gradient-echo-based cine pulse sequence, and the animals were monitored by respiration and pulse oximetry. The analysis revealed an increase in the thickness of the ventricular wall in EAM mice, with a corresponding decrease in the interior diameter of ventricles, when compared with healthy mice. The data suggest that morphological and functional changes in the inflamed hearts can be non-invasively monitored by MRM in live animals. In conclusion, MRM offers an advantage of assessing the progression and regression of myocardial injuries in diseases caused by infectious agents, as well as response to therapies.
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