In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.
In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.
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DOI：
10.1016/j.devcel.2014.03.007
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发表时间：
2014-04-28
影响因子：
11.8
通讯作者：
Maddox, Amy S.
中科院分区：
文献类型：
--
作者：
Lacroix, Benjamin;Bourdages, Karine G.;Dorn, Jonas F.;Ihara, Shinji;Sherwood, David R.;Maddox, Paul S.;Maddox, Amy S.
微管（MT）是细胞骨架聚合物，经历动态不稳定性，生长和收缩阶段之间的随机过渡。MT动力学是多种细胞过程所需的，并且虽然是微管蛋白固有的，但受到高度调节。然而，很少有人知道MT动力学如何促进或调节组织的生物发生和分化。我们在完整发育的C.优雅的。MT动力学的各个方面都发生了显着变化，茎样前体退出有丝分裂，其次，因为他们分化。我们发现，抑制，而不是增强动力学扰动分化肌肉功能在体内。MT相关蛋白的不同集合体是组织生物发生与组织功能特别需要的。CLASP家族MT稳定剂和解聚驱动蛋白MCAK分别是前体细胞或分化细胞中MT动力学所需的差异。所有这些多维表型比较都是通过一种新的数据显示方法，菱形图来进行的。
Microtubules (MTs) are cytoskeletal polymers that undergo dynamic instability, the stochastic transition between growth and shrinkage phases. MT dynamics are required for diverse cellular processes, and, while intrinsic to tubulin, are highly regulated. However, little is known about how MT dynamics facilitate or are regulated by tissue biogenesis and differentiation. We imaged MT dynamics in a smooth muscle-like lineage in intact developing C. elegans. All aspects of MT dynamics change significantly as stem-like precursors exit mitosis and, secondarily, as they differentiate. We found that suppression but not enhancement of dynamics perturbs differentiated muscle function in vivo. Distinct ensembles of MT-associated proteins are specifically required for tissue biogenesis versus tissue function. A CLASP family MT stabilizer and the depolymerizing kinesin MCAK are differentially required for MT dynamics in the precursor or differentiated cells, respectively. All these multidimensional phenotypic comparisons were facilitated by a novel data display method, the diamond graph.
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