Directed self-assembly of fluorescence responsive nanoparticles and their use for real-time surface and cellular imaging.
Directed self-assembly of fluorescence responsive nanoparticles and their use for real-time surface and cellular imaging.
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DOI：
10.1038/s41467-017-02060-8
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发表时间：
2017-12-01
影响因子：
16.6
通讯作者：
O'Shea DF
中科院分区：
文献类型：
--
作者：
Cheung S;O'Shea DF
水中定向自组装被认为是自然界中形成复杂的高级有序结构的最有效手段。在这里，我们展示了一种简单而强大的颗粒组装方法，该方法利用两亲性三嵌段共聚物泊洛沙姆-188和疏水性荧光团作为两个设计师组件，它们具有内置的能力，可以向观察者传达有关其周围环境的空间和时间信息。粒子自组装的模板作用归因于荧光团与泊洛沙姆疏水链段之间的相互作用。水中的颗粒荧光被淬灭，但可以被诱导以响应于温度、表面吸附和细胞摄取而选择性地打开。颗粒动态调节发射强度的能力可用于药物晶体表面、天然纤维和胰岛素原纤维的选择性标记和实时成像以及细胞递送。由于颗粒溶液容易制备，这种水基近红外荧光涂料的进一步应用是可以预期的。具有在药物递送期间引起输出的内置能力的聚合物纳米颗粒是非常期望的。在这里，作者描述了一种两亲性三嵌段共聚物的自组装，该共聚物显示出响应于温度、表面吸附和细胞摄取的颗粒荧光。
Directed self-assemblies in water are known as the most efficient means of forming complex higher ordered structures in nature. Here we show a straightforward and robust method for particle assembly which utilises the amphiphilic tri-block co-polymer poloxamer-188 and a hydrophobic fluorophore as the two designer components, which have a built-in ability to convey spatial and temporal information about their surroundings to an observer. Templating of particle self-assembly is attributed to interactions between the fluorophore and hydrophobic segment of the poloxamer. Particle fluorescence in water is quenched but can be induced to selectively switch on in response to temperature, surface adsorption and cellular uptake. The ability of the particles to dynamically modulate emission intensity can be exploited for selective labelling and real-time imaging of drug crystal surfaces, natural fibres and insulin fibrils, and cellular delivery. As particle solutions are easily prepared, further applications for this water-based NIR-fluorescent paint are anticipated. Polymer nanoparticles that have an in-built capacity to elicit an output during drug delivery are highly desirable. Here the authors describe the self-assembly of an amphiphilic tri-block co-polymer that shows particle fluorescence in response to temperature, surface adsorption and cellular uptake.
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