Identification of miRNAs during mouse postnatal ovarian development and superovulation.
Identification of miRNAs during mouse postnatal ovarian development and superovulation.
复制标题
小鼠产后卵巢发育和超数排卵过程中 miRNA 的鉴定
DOI：
10.1186/s13048-015-0170-2
复制
发表时间：
2015-07-08
影响因子：
4
通讯作者：
Huo LJ
中科院分区：
文献类型：
--
作者：
Khan HA;Zhao Y;Wang L;Li Q;Du YA;Dan Y;Huo LJ
背景MicroRNA是一类小分子非编码RNA，在转录后水平上通过序列互补性调控卵巢等多种组织的基因表达。这些miRNAs可在转录水平上对参与卵巢发育和卵泡形成的众多基因进行调控。因此，组织特异性miRNAs的鉴定被认为是理解miRNAs在生物过程中的作用的关键一步。MethodsTo研究microRNAs在卵巢发育和卵泡形成过程中的作用，我们使用Illumina深度测序技术对8个不同的文库进行测序。结果从大量测序结果中筛选出16-26 bp的清晰片段，用于差异表达分析和microRNA注释。对所有miRNAs在不同发育阶段的表达分析表明，一些miRNAs普遍存在，而另一些则在不同阶段差异表达。在差异表达的miRNAs中，我们报道了61个miRNAs在所有文库中不同发育阶段的倍数变化超过2。在上调的miRNAs中，mmu-mir-1298具有最高的倍数变化，为4.025，而mmu-mir-150下调超过3倍。此外，我们使用七种不同的靶标预测程序（DIANA-mT，米兰达，miRDB，miRWalk，RNAhybrid，PICTAR 5，TargetScan）发现了20种差异表达的microRNA的2659个靶基因。对预测靶点的分析显示，某些卵巢特异性基因被单个或多个microRNA靶向。此外，途径注释和基因本体论表明，这些microRNAs参与基本的细胞processing.ConclusionsThese结果表明，不同的miRNA在卵巢发育和超排卵的不同阶段的存在。利用生物信息学工具阐明了这些microRNA在调节卵巢发育和超数排卵的不同途径、生物学功能和细胞组分中的潜在作用。这些结果为进一步分析miRNAs及其在卵巢发育和超数排卵中的作用提供了框架。此外，这项研究为单个miRNAs的表征提供了基础，以发现它们在卵巢发育和女性生育力中的作用。
BackgroundMicroRNAs are small noncoding RNAs that play critical roles in regulation of gene expression in wide array of tissues including the ovary through sequence complementarity at post-transcriptional level. Tight regulation of multitude of genes involved in ovarian development and folliculogenesis could be regulated at transcription level by these miRNAs. Therefore, tissue specific miRNAs identification is considered a key step towards understanding the role of miRNAs in biological processes.MethodsTo investigate the role of microRNAs during ovarian development and folliculogenesis we sequenced eight different libraries using Illumina deep sequencing technology. Different developmental stages were selected to explore miRNAs expression pattern at different stages of gonadal maturation with/without treatment of PMSG/hCG for superovulation.ResultsFrom massive sequencing reads, clean reads of 16–26 bp were selected for further analysis of differential expression analysis and novel microRNA annotation. Expression analysis of all miRNAs at different developmental stages showed that some miRNAs were present ubiquitously while others were differentially expressed at different stages. Among differentially expressed miRNAs we reported 61 miRNAs with a fold change of more than 2 at different developmental stages among all libraries. Among the up-regulated miRNAs, mmu-mir-1298 had the highest fold change with 4.025 while mmu-mir-150 was down-regulated more than 3 fold. Furthermore, we found 2659 target genes for 20 differentially expressed microRNAs using seven different target predictions programs (DIANA-mT, miRanda, miRDB, miRWalk, RNAhybrid, PICTAR5, TargetScan). Analysis of the predicted targets showed certain ovary specific genes targeted by single or multiple microRNAs. Furthermore, pathway annotation and Gene ontology showed involvement of these microRNAs in basic cellular process.ConclusionsThese results suggest the presence of different miRNAs at different stages of ovarian development and superovulation. Potential role of these microRNAs was elucidated using bioinformatics tools in regulation of different pathways, biological functions and cellular components underlying ovarian development and superovulation. These results provide a framework for extended analysis of miRNAs and their roles during ovarian development and superovulation. Furthermore, this study provides a base for characterization of individual miRNAs to discover their role in ovarian development and female fertility.
登录
查看更多内容
影响因子：
3.6
作者：
Juanchich, Amelie;Le Cam, Aurelie;Bobe, Julien
通讯作者：
Bobe, Julien
影响因子：
3.6
作者：
Carletti, Martha Z.;Fiedler, Stephanie D.;Christenson, Lane K.
通讯作者：
Christenson, Lane K.
影响因子：
3.6
作者：
Fiedler, Stephanie D.;Carletti, Martha Z.;Christenson, Lane K.
通讯作者：
Christenson, Lane K.
影响因子：
14.9
作者：
Betel D;Wilson M;Gabow A;Marks DS;Sander C
通讯作者：
Sander C
影响因子：
9.2
作者：
Huang J;Ju Z;Li Q;Hou Q;Wang C;Li J;Li R;Wang L;Sun T;Hang S;Gao Y;Hou M;Zhong J
通讯作者：
Zhong J