High-order oligomerization is required for the function of the H-NS family member MvaT in Pseudomonas aeruginosa.
High-order oligomerization is required for the function of the H-NS family member MvaT in Pseudomonas aeruginosa.
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DOI：
10.1111/j.1365-2958.2010.07378.x
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发表时间：
2010-11
影响因子：
3.6
通讯作者：
Dove SL
中科院分区：
文献类型：
--
作者：
Castang S;Dove SL
H-NS 是一种丰富的 DNA 结合蛋白，与几种不同细菌中外源 DNA 的沉默有关。 H-NS 二聚体形成高阶寡聚体的能力被认为有助于蛋白质在富含 AT 的 DNA 片段上聚合，并促进基因沉默。尽管来自肠道细菌的 H-NS 寡聚化一直是深入研究的主题，但对于来自肠杆菌科以外的细菌的 H-NS 家族成员的寡聚化知之甚少，其中许多细菌与其肠道对应物几乎没有序列相似性。在这里，我们表明，MvaT（铜绿假单胞菌蛋白质 H-NS 家族的成员）可以形成二聚体和高阶寡聚物，并且我们确定了 MvaT 内介导高阶寡聚物形成的区域。通过基因检测，我们鉴定出存在高阶寡聚物形成缺陷的 MvaT 突变体。我们提供的证据表明，这些突变体由于无法形成高阶寡聚体而功能受损并表现出 DNA 结合缺陷。我们的研究结果支持这样一个模型：MvaT 与 DNA 有效结合的能力取决于 MvaT 二聚体之间的蛋白质-蛋白质相互作用，并表明形成更高阶寡聚体的能力是 H-NS 家族成员的保守且基本的特征。
H-NS is an abundant DNA-binding protein that has been implicated in the silencing of foreign DNA in several different bacteria. The ability of H-NS dimers to form higher-order oligomers is thought to aid the polymerization of the protein across AT-rich stretches of DNA and facilitate gene silencing. Although the oligomerization of H-NS from enteric bacteria has been the subject of intense investigation, little is known regarding the oligomerization of H-NS family members from bacteria outside of the enterobacteriaceae, many of which share little sequence similarity with their enteric counterparts. Here we show that MvaT, a member of the H-NS family of proteins from Pseudomonas aeruginosa, can form both dimers and higher order oligomers, and we identify a region within MvaT that mediates higher-order oligomer formation. Using genetic assays we identify mutants of MvaT that are defective for higher-order oligomer formation. We present evidence that these mutants are functionally impaired and exhibit DNA-binding defects because of their inability to form higher-order oligomers. Our findings support a model in which the ability of MvaT to bind efficiently to the DNA depends upon protein-protein interactions between MvaT dimers and suggest that the ability to form higher-order oligomers is a conserved and essential feature of H-NS family members.
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影响因子：
14.9
作者：
Lang B;Blot N;Bouffartigues E;Buckle M;Geertz M;Gualerzi CO;Mavathur R;Muskhelishvili G;Pon CL;Rimsky S;Stella S;Babu MM;Travers A
通讯作者：
Travers A
影响因子：
4.8
作者：
Hu, JC;Kornacker, MG;Hochschild, A
通讯作者：
Hochschild, A
影响因子：
3.6
作者：
FALCONI, M;GUALTIERI, MT;PON, CL
通讯作者：
PON, CL
影响因子：
3.2
作者：
Dame, RT;Luijsterburg, MS;Wuite, GJL
通讯作者：
Wuite, GJL
影响因子：
3.2
作者：
Li, Congran;Wally, Hassan;Lu, Chung-Dar
通讯作者：
Lu, Chung-Dar