Precision-porous templated scaffolds of varying pore size drive dendritic cell activation.
Precision-porous templated scaffolds of varying pore size drive dendritic cell activation.
复制标题
DOI：
10.1002/bit.26532
复制
发表时间：
2018-04
影响因子：
3.8
通讯作者：
Bryers JD
中科院分区：
文献类型：
--
作者：
Chen R;Ma H;Zhang L;Bryers JD
基于支架的系统在调节体内免疫反应方面显示出巨大的潜力。虽然巨噬细胞与用于组织再生的组织工程支架的相互作用受到了广泛关注，但很少有研究关注支架设计对免疫细胞（即树突状细胞（DC））反应的影响。在这里，我们展示了聚（甲基丙烯酸 2-羟乙酯）（pHEMA）和聚（二甲基硅氧烷）（PDMS，有机硅）支架的不同孔径对 DC 的成熟和体内富集的影响。我们采用精密模板方法来制作具有统一定义和可调节架构的 3D 多孔聚合物支架。亲水性 pHEMA 和疏水性 PDMS 支架被制成三种孔径（20、40、90 μm），以量化支架孔径对体外和体内 DC 活化/成熟的影响。体外结果表明，pHEMA 和 PDMS 支架均可促进 DC 细胞系 JAWSII 的成熟，该细胞系类似于脂多糖 (LPS) 激活/成熟的 DC (mDC)。无论使用何种聚合物，孔径较小的支架与较高的 DC 成熟度相关。在体内，当皮下植入 C57BL/6J 小鼠时，孔径较小的支架也表现出更多的 DC 募集和更持续的激活。在不使用 DC 化学引诱剂或化学佐剂的情况下，我们的结果表明，可以通过简单地改变支架的孔径来调节 DC 成熟和支架渗透特征。
Scaffold based systems have shown significant potential in modulating immune responses in vivo. While there has been much attention on macrophage interactions with tissue engineered scaffolds for tissue regeneration, fewer studies have looked at the effects of scaffold design on the response of immune cells−that is, dendritic cells (DCs). Here, we present the effects of varying pore size of poly (2-hydroxyethyl methacrylate) (pHEMA) and poly(dimethylsiloxane) (PDMS, silicone) scaffolds on the maturation and in vivo enrichment of DCs. We employ a precision templating method to make 3-D porous polymer scaffolds with uniformly defined and adjustable architecture. Hydrophilic pHEMA and hydrophobic PDMS scaffolds were fabricated in three pore sizes (20, 40, 90 μm) to quantify scaffold pore size effects on DCs activation/maturation in vitro and in vivo. In vitro results showed that both pHEMA and PDMS scaffolds could promote maturation in the DC cell line, JAWSII, that resembled lipopolysaccharide (LPS)-activated/matured DCs (mDCs). Scaffolds with smaller pore sizes correlate with higher DC maturation, regardless of the polymer used. In vivo, when implanted subcutaneously in C57BL/6J mice, scaffolds with smaller pore sizes also demonstrated more DCs recruitment and more sustained activation. Without the use of DC chemo-attractants or chemical adjuvants, our results suggested that DC maturation and scaffold infiltration profile can be modulated by simply altering the pore size of the scaffolds.
登录
查看更多内容
影响因子：
10.8
作者：
Ali, Omar A.;Mooney, David J.
通讯作者：
Mooney, David J.
DOI：
10.1038/nrc3258
发表时间：
2012-03-22
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
7.4
作者：
Andorko JI;Jewell CM
通讯作者：
Jewell CM
影响因子：
4.9
作者：
Babensee, JE;Paranjpe, A
通讯作者：
Paranjpe, A
DOI：
10.1073/pnas.1006442107
发表时间：
2010-08-24
影响因子：
11.1
作者：
Madden, Lauran R.;Mortisen, Derek J.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.