"On-off" thermoresponsive coating agent containing salicylic acid applied to maize seeds for chilling tolerance.
"On-off" thermoresponsive coating agent containing salicylic acid applied to maize seeds for chilling tolerance.
复制标题
– 开关 – 含有水杨酸的温敏包衣剂应用于玉米种子的耐冷性
DOI：
10.1371/journal.pone.0120695
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
Guan Y;Li Z;He F;Huang Y;Song W;Hu J
低温胁迫是限制玉米种子田间结实的重要因素。在这项研究中，一种“开-关”型温敏涂层剂含有聚（N-异丙基丙烯酰胺-co-甲基丙烯酸丁酯）（缩写。为提高玉米种子的耐冷性，研制了P（NIPAm-co-BMA）水凝胶。以N-异丙基丙烯酰胺（NIPAm）和甲基丙烯酸丁酯（BMA）为单体，通过自由基聚合制备了P（NIPAm-co-BMA）水凝胶。将水杨酸（SA）作为抗冷剂负载到水凝胶中。以黄C（HC）和Mo 17（冷敏感）两个玉米品种为试材，研究了SA负载P（NIPAm-co-BMA）对低温胁迫下玉米种子萌发和幼苗生长的影响。结果显示，水凝胶获得接近12°C的相变温度，其中NIPAM与MBA的重量比为1：0.1988（w/w）。12°C的温度被认为是耐冷剂释放的“开-关”温度; SA在低于12 ° C的外部温度下比在高于12°C的外部温度下更快地从水凝胶释放。此外，当两个玉米品种的幼苗遭受短期低温胁迫（5°C）时，与单一SA处理相比，较高浓度的SA负载水凝胶导致包衣玉米种子的发芽势、发芽率、发芽指数、根长、苗高、根和苗的干重以及保护酶活性增加，丙二醛含量降低。与对照组相比，这些生理和生化参数中的大多数都达到了显着水平。因此，SA-负载P（NIPAm-co-BMA）是一种无毒的温敏性水凝胶，可作为提高玉米种子耐冷性的有效材料。
Chilling stress is an important constraint for maize seed establishment in the field. In this study, a type of “on-off” thermoresponsive coating agent containing poly (N-isopropylacrylamide-co-butylmethacrylate) (Abbr. P(NIPAm-co-BMA)) hydrogel was developed to improve the chilling tolerance of coated maize seed. The P(NIPAm-co-BMA) hydrogel was synthesized by free-radical polymerization of N-isopropylacrylamide (NIPAm) and butylmethacrylate (BMA). Salicylic acid (SA) was loaded in the hydrogel as the chilling resistance agent. SA-loaded P(NIPAm-co-BMA) was used for seed film-coating of two maize varieties, Huang C (HC, chilling-tolerant) and Mo17 (chilling-sensitive), to investigate the coated seed germination and seedling growth status under chilling stress. The results showed that the hydrogel obtained a phase transition temperature near 12°C with a NIPAM to MBA weight ratio of 1: 0.1988 (w/w). The temperature of 12°C was considered the “on-off” temperature for chilling-resistant agent release; the SA was released from the hydrogel more rapidly at external temperatures below 12°C than above 12°C. In addition, when seedlings of both maize varieties suffered a short chilling stress (5°C), higher concentrations of SA-loaded hydrogel resulted in increased germination energy, germination percentage, germination index, root length, shoot height, dry weight of roots and shoots and protective enzyme activities and a decreased malondialdehyde content in coated maize seeds compared to single SA treatments. The majority of these physiological and biochemical parameters achieved significant levels compared with the control. Therefore, SA-loaded P(NIPAm-co-BMA), a nontoxic thermoresponsive hydrogel, can be used as an effective material for chilling tolerance in film-coated maize seeds.
登录
查看更多内容
影响因子：
4.3
作者：
Cao, D. D.;Hu, J.;Knapp, A.
通讯作者：
Knapp, A.
影响因子：
5.8
作者：
Makino, K;Hiyoshi, J;Ohshima, H
通讯作者：
Ohshima, H
影响因子：
4.3
作者：
Gunes, Aydin;Inal, Ali;Cicek, Nuray
通讯作者：
Cicek, Nuray
影响因子：
4.2
作者：
Gao, Canhong;Hu, Jin;Knapp, Allen
通讯作者：
Knapp, Allen
影响因子：
4.3
作者：
Janda, T;Szalai, G;Páldi, E
通讯作者：
Páldi, E