Estimating Human Trabecular Meshwork Stiffness by Numerical Modeling and Advanced OCT Imaging.
Estimating Human Trabecular Meshwork Stiffness by Numerical Modeling and Advanced OCT Imaging.
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DOI：
10.1167/iovs.17-22175
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发表时间：
2017-09-01
影响因子：
4.4
通讯作者：
Ethier CR
中科院分区：
文献类型：
--
作者：
Wang K;Johnstone MA;Xin C;Song S;Padilla S;Vranka JA;Acott TS;Zhou K;Schwaner SA;Wang RK;Sulchek T;Ethier CR
本研究的目的是使用一种新的间接方法来估计青光眼患者小梁网（hTM）硬度升高，并将结果与直接面原子力显微镜（AFM）测量结果进行比较。死后人眼灌注测量流出设施，并通过示踪剂识别高流量和低流量区域（HF， LF）。直接操纵施勒姆管腔压，获得光学相干断层扫描（OCT）图像。采用有限元逆建模方法推导了TM的刚度。一系列的AFM力图沿着一条线穿过前角的径向切割平装角骨楔，TM面朝上。正常hTM弹性模量为70±20 kPa (mean±SD)，青光眼hTM弹性模量略偏硬（98±19 kPa）。这一趋势与AFM测量的TM刚度一致：正常hTM刚度= 1.37±0.56 kPa，低于青光眼hTM刚度（2.75±1.19 kPa）。这些差异都没有统计学意义。反有限元分析结果表明，正常眼和青光眼HF楔体的TM比LF楔体软，AFM分析结果则相反。6只人眼的流出设施与FEM估计的TM刚度显著相关（P = 0.018）。青光眼患者的TM僵硬度较高，但仅为适度。正常人和青光眼的流出设施似乎与TM僵硬有关。这一证据激发了进一步研究TM生物力学特性调控的潜在因素。
The purpose of this study was to estimate human trabecular meshwork (hTM) stiffness, thought to be elevated in glaucoma, using a novel indirect approach, and to compare results with direct en face atomic force microscopy (AFM) measurements. Postmortem human eyes were perfused to measure outflow facility and identify high- and low-flow regions (HF, LF) by tracer. Optical coherence tomography (OCT) images were obtained as Schlemm's canal luminal pressure was directly manipulated. TM stiffness was deduced by an inverse finite element modeling (FEM) approach. A series of AFM forcemaps was acquired along a line traversing the anterior angle on a radially cut flat-mount corneoscleral wedge with TM facing upward. The elastic modulus of normal hTM estimated by inverse FEM was 70 ± 20 kPa (mean ± SD), whereas glaucomatous hTM was slightly stiffer (98 ± 19 kPa). This trend was consistent with TM stiffnesses measured by AFM: normal hTM stiffness = 1.37 ± 0.56 kPa, which was lower than glaucomatous hTM stiffness (2.75 ± 1.19 kPa). None of these differences were statistically significant. TM in HF wedges was softer than that in LF wedges for both normal and glaucomatous eyes based on the inverse FEM approach but not by AFM. Outflow facility was significantly correlated with TM stiffness estimated by FEM in six human eyes (P = 0.018). TM stiffness is higher, but only modestly so, in glaucomatous patients. Outflow facility in both normal and glaucomatous human eyes appears to associate with TM stiffness. This evidence motivates further studies to investigate factors underlying TM biomechanical property regulation.
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影响因子：
3.4
作者：
Allingham, RR;deKater, AW;Ethier, CR
通讯作者：
Ethier, CR
影响因子：
3.4
作者：
Honjo, M;Inatani, M;Tanihara, H
通讯作者：
Tanihara, H
影响因子：
4.2
作者：
JOHNSTONE, MA
通讯作者：
JOHNSTONE, MA
影响因子：
3.5
作者：
Nguyen, T. D.;Boyce, B. L.
通讯作者：
Boyce, B. L.
DOI：
10.1007/bf00421587
发表时间：
1968-01-01
期刊：
Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology
影响因子：
--
作者：
Rohen, J W;Rentsch, F J
通讯作者：
Rentsch, F J