Imaging of reactive oxygen species generated in vivo
Imaging of reactive oxygen species generated in vivo
复制标题
体内产生的活性氧的成像
作者：
Togashi H;et al.
目的：研究人员使用一种体内电子自旋共振（ESR）成像系统，利用自旋探针，1‐乙酰氧基‐3‐氨基甲酰‐2,2,5,5‐四甲基吡咯啉（ACP）与活性氧反应后产生ESR可检测的氮氧化物，试图对活性氧（ROS）在活体中的生物分布进行成像。方法采用热杀灭小棒状杆菌诱导小鼠急性肝损伤，然后注射低剂量脂多糖。ACP静脉注射，并使用体内ESR成像系统显示肝脏氧化应激。结果在免疫介导的肝损伤模型中，脂多糖给药后3 h，大鼠出现明显的氧化应激。当观察到明显的肝脏氧化应激时，在脂多糖注射后3小时静脉给予ACP， ESR成像系统检测到3‐氨基甲酰‐2,2,5,5‐四甲基吡咯烷醚（carbamoyl‐PROXYL）的高信号，该物质来源于ACP衍生的羟胺，并在活体中产生大量ROS。使用带ACP的ESR成像系统，我们能够在肝损伤发生前看到腹部的ROS。结论：我们已经成功地在器官损伤发生前可视化体内的活性氧，这代表了有毒分子成像的重大发展。中华医学杂志，2016,25(5):557 - 557。©2015 Wiley期刊公司
PurposeWe sought to image the biodistribution of reactive oxygen species (ROS) within the living body using an in vivo electron spin resonance (ESR) imaging system using a spin probe, 1‐acetoxy‐3‐carbamoyl‐2,2,5,5‐tetramethylpyrroline (ACP) that produces ESR‐detectable nitroxide upon reaction with ROS.MethodsAcute hepatic injury was induced in mice by priming with heat‐killedCorynebacterium parvumfollowed by injection of a low dose of lipopolysaccharide. ACP was administered intravenously and an in vivo ESR imaging system was used to visualize hepatic oxidative stress.ResultsIn this immune‐mediated hepatic injury model, significant oxidative stress was evident at 3 h after lipopolysaccharide administration before the onset of massive hepatic injury. ACP was administered intravenously at 3 h after lipopolysaccharide injection when significant hepatic oxidative stress had been observed, and the ESR imaging system detected a high signal for 3‐carbamoyl‐2,2,5,5‐tetramethylpyrrolidine (carbamoyl‐PROXYL), which had originated from the ACP‐derived hydroxylamine and produced large amount of ROS within the living body. Using the ESR imaging system with ACP, we were able to visualize ROS in the abdomen before onset of hepatic injury.ConclusionWe have succeeded in visualizing ROS within the body before onset of organ damage, representing a significant development in imaging for toxic molecules. Magn Reson Med 75:1375–1379, 2016. © 2015 Wiley Periodicals, Inc.
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DOI：
--
发表时间：
2008
期刊：
Gastroenterology (in press)
影响因子：
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DOI：
10.1007/bf02773878
发表时间：
1989
期刊：
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影响因子：
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作者：
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影响因子：
7.4
作者：
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通过电子自旋共振计算机断层扫描对肝脏氧化应激增加进行体内成像。
DOI：
--
发表时间：
2003
期刊：
Research Communications in Molecular Pathology and Pharmacology
影响因子：
--
作者：
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2.5
作者：
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通讯作者：
Takahashi, T