Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis.
Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis.
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DOI：
10.1371/journal.pone.0011724
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发表时间：
2010-07-22
期刊：
影响因子：
3.7
通讯作者：
Santiviago CA
中科院分区：
文献类型：
--
作者：
Blondel CJ;Yang HJ;Castro B;Chiang S;Toro CS;Zaldívar M;Contreras I;Andrews-Polymenis HL;Santiviago CA
鸡沙门氏菌是一种具有家禽特异性宿主范围的病原体，而肠道沙门氏菌是一种广泛宿主范围的病原体，其亚临床地定殖家禽，但却是人类和许多其他物种中胃肠道沙门氏菌病的主要原因。尽管最近的进展，我们了解沙门氏菌和它们的宿主之间的复杂的相互作用，宿主范围限制和独特的病理生物学鸡的分子基础仍然在很大程度上未知。VI型分泌系统（T6 SS）代表了一种新的蛋白质分泌模式，对许多革兰氏阴性菌的发病机制至关重要。我们最近在鸡沙门氏菌致病岛19（SPI-19）中鉴定了一个假定的T6 SS。在肠道病毒中，SPI-19是一种退化的元件，它已经失去了岛上编码的大部分T6 SS功能。在这项工作中，我们研究了SPI-19对鸡沙门氏菌菌株287/91在鸡体内定殖的贡献。SPI-19和clpV基因的非极性缺失突变体，一个必要的T6 SS组件，定植回肠，盲肠，肝脏和脾脏的白色来航鸡口服接种后相比，野生型菌株。使用VEX-Capture将SPI-19返回到ΔSPI-19突变体，补充了这种定殖缺陷。相比之下，SPI-19从鸡转移到肠杆菌导致在感染后第1天回肠、肝脏和脾脏的定殖短暂增加，但在感染后第3天和第5天观察到实验感染鸡的肠道和内脏的强烈定殖缺陷。我们的数据表明，SPI-19和T6 SS编码在这个区域有助于鸡沙门氏菌的胃肠道和内脏器官的殖民化，并建议肠道沙门氏菌的SPI-19 T6 SS的降解赋予了优势，在鸟类宿主的殖民化。
Salmonella Gallinarum is a pathogen with a host range specific to poultry, while Salmonella Enteritidis is a broad host range pathogen that colonizes poultry sub-clinically but is a leading cause of gastrointestinal salmonellosis in humans and many other species. Despite recent advances in our understanding of the complex interplay between Salmonella and their hosts, the molecular basis of host range restriction and unique pathobiology of Gallinarum remain largely unknown. Type VI Secretion System (T6SS) represents a new paradigm of protein secretion that is critical for the pathogenesis of many Gram-negative bacteria. We recently identified a putative T6SS in the Salmonella Pathogenicity Island 19 (SPI-19) of Gallinarum. In Enteritidis, SPI-19 is a degenerate element that has lost most of the T6SS functions encoded in the island. In this work, we studied the contribution of SPI-19 to the colonization of Salmonella Gallinarum strain 287/91 in chickens. Non-polar deletion mutants of SPI-19 and the clpV gene, an essential T6SS component, colonized the ileum, ceca, liver and spleen of White Leghorn chicks poorly compared to the wild-type strain after oral inoculation. Return of SPI-19 to the ΔSPI-19 mutant, using VEX-Capture, complemented this colonization defect. In contrast, transfer of SPI-19 from Gallinarum to Enteritidis resulted in transient increase in the colonization of the ileum, liver and spleen at day 1 post-infection, but at days 3 and 5 post-infection a strong colonization defect of the gut and internal organs of the experimentally infected chickens was observed. Our data indicate that SPI-19 and the T6SS encoded in this region contribute to the colonization of the gastrointestinal tract and internal organs of chickens by Salmonella Gallinarum and suggest that degradation of SPI-19 T6SS in Salmonella Enteritidis conferred an advantage in colonization of the avian host.
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影响因子：
3.1
作者：
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通讯作者：
Barrow, PA
影响因子：
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作者：
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作者：
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DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
DOI：
10.1073/pnas.0813360106
发表时间：
2009-03-17
影响因子：
11.1
作者：
Leiman, Petr G.;Basler, Marek;Mekalanos, John J.
通讯作者：
Mekalanos, John J.