Aerosols modification with H(2)O(2) reduces airborne contamination by dental handpieces.
Aerosols modification with H(2)O(2) reduces airborne contamination by dental handpieces.
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DOI：
10.1080/20002297.2021.1881361
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发表时间：
2021-01-29
影响因子：
4.5
通讯作者：
Rizzo R
中科院分区：
文献类型：
--
作者：
Ionescu AC;Brambilla E;Manzoli L;Orsini G;Gentili V;Rizzo R
目的：我们设计了一项体外研究，以评估0.5 vol%过氧化氢喷雾剂在传统牙科手术中减少冠状病毒229 E传播的效率。方法：利用人体模型为患者和操作者定制一个III类柜室。将HCoV-229 E在人工唾液中的悬浮液接种到患者的模型口中，该悬浮液具有与SARS-CoV-2无症状患者相似的病毒载量。使用气溶胶生成空气涡轮机进行持续10 s的牙科手术，有或没有高容量抽空（HVE）。测试了0.5体积% H2 O2冷却喷雾在降低病毒载量方面的效果。通过面罩外表面、体模前额和口腔内的实时定量PCR评估操作员体模上的病毒存在。结果如下：当使用H2 O2冷却喷雾时，与常规喷雾相比，所有检测部位的病毒载量均显著降低，低于检测限。当使用HVE时，在任何试验部位中病毒载量均未发生显著变化。结论：牙科手机使用0.5 vol% H2 O2冷却喷雾大大降低了在气溶胶生成牙科手术期间冠状病毒传播的可能性。在SARS-CoV-2大流行期间采取的预防措施中，这一战略值得进一步考虑。
Objective: We designed an in vitro study to evaluate the efficiency of an 0.5 vol% hydrogen peroxide-based spray in reducing Coronavirus 229E spread during a conventional dental procedure. Methods: A class III cabinet-like chamber was custom-built, using phantoms for both patient and operator. A suspension of HCoV-229E in artificial saliva having a similar viral load to SARS-CoV-2 asymptomatic patients was inoculated inside the patient’s phantom mouth. A 10 s-lasting dental procedure was performed using an aerosol-generating air-turbine, with or without high-volume evacuation (HVE). The effect of 0.5 vol% H2O2 cooling spray in reducing viral loads was tested. Viral presence on the operator phantom was assessed by Real-Time quantitative PCR on the mask’s outer surface, on the phantom’s forehead, and inside its mouth. Results: When the H2O2 cooling spray was used, as compared to the conventional spray, viral loads were significantly lower on all tested sites, falling below the detection limit. Viral loads did not significantly change in any tested site when HVE was used. Conclusion: The use of 0.5 vol% H2O2 cooling spray by dental handpieces drastically reduced the possibility of coronaviruses spread during aerosol-generating dental procedures. This strategy deserves further consideration among the preventive measures to be adopted during the SARS-CoV-2 pandemic.
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