The combined effect of electrical stimulation and high-load isometric contraction on protein degradation pathways in muscle atrophy induced by hindlimb unloading.
The combined effect of electrical stimulation and high-load isometric contraction on protein degradation pathways in muscle atrophy induced by hindlimb unloading.
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DOI：
10.1155/2011/401493
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发表时间：
2011
影响因子：
--
通讯作者：
Fujino H
中科院分区：
文献类型：
--
作者：
Fujita N;Murakami S;Fujino H
大负荷等长运动被认为是对抗肌肉萎缩的有效对策，但治疗肌肉萎缩的电刺激通常是在没有负荷的情况下进行的。在本研究中，我们研究了电刺激和高负荷等长收缩在预防后肢卸载所致肌肉萎缩方面的联合效果。无负荷电刺激可使肌肉萎缩程度轻微减轻。此外，将电刺激与高负荷等长收缩相结合，增强了这种效应。在不加负荷的电刺激中，Calain 1、calain 2和Murf-1mRNA的过度表达受到抑制。另一方面，在高负荷等长收缩的电刺激中，除了抑制钙蛋白酶1、钙蛋白酶2和MURF-1mRNA的过度表达外，组织蛋白酶L和阿托金-1mRNA的过度表达也受到抑制。这些发现表明，电刺激和高负荷等长收缩相结合是有效的对抗肌肉萎缩的方法。
High-load isometric exercise is considered an effective countermeasure against muscle atrophy, but therapeutic electrical stimulation for muscle atrophy is often performed without loading. In the present study, we investigated the combined effectiveness of electrical stimulation and high-load isometric contraction in preventing muscle atrophy induced by hindlimb unloading. Electrical stimulation without loading resulted in slight attenuation of muscle atrophy. Moreover, combining electrical stimulation with high-load isometric contraction enhanced this effect. In electrical stimulation without loading, inhibition of the overexpression of calpain 1, calpain 2, and MuRF-1 mRNA was confirmed. On the other hand, in electrical stimulation with high-load isometric contraction, inhibition of the overexpression of cathepsin L and atrogin-1 mRNA in addition to calpain 1, calpain 2, and MuRF-1 mRNA was confirmed. These findings suggest that the combination of electrical stimulation and high-load isometric contraction is effective as a countermeasure against muscle atrophy.
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DOI：
10.1097/phm.0b013e31818e02d6
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