Microsatellite Polymorphisms Adjacent to the Oxytocin Receptor Gene in Domestic Cats: Association with Personality?
Microsatellite Polymorphisms Adjacent to the Oxytocin Receptor Gene in Domestic Cats: Association with Personality?
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DOI：
10.3389/fpsyg.2017.02165
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发表时间：
2017
影响因子：
3.8
通讯作者：
Fujita K
中科院分区：
文献类型：
--
作者：
Arahori M;Chijiiwa H;Takagi S;Bucher B;Abe H;Inoue-Murayama M;Fujita K
越来越多的研究探索了人类和非人类动物的催产素系统，其中一些研究发现催产素受体基因（OXTR）中存在重要的遗传多态性，这些多态性与多个物种的联结系统、社会行为和个性相关。尽管 OXTR 中的单核苷酸多态性已在不同物种中得到充分研究，但与 OXTR 相邻的微卫星（或短串联重复序列）却很少被研究，尽管有一些建议表明基因附近的微卫星多态性可能在遗传转录和翻译中发挥作用。在这项研究中，我们调查了家猫（Felis catus）OXTR 上游、内含子和下游区域的微卫星。我们成功地扩增了 10 个区域中的 5 个，并将这 5 个区域识别为多态性的。我们比较了日本杂种猫 (n = 100) 和 10 个纯种猫 (n = 40) 在这五个地区的等位基因频率。两个群体在所有微卫星区域的等位基因频率均存在显着差异。此外，杂种猫的主人还回答了一份全面的性格调查问卷，并通过因子分析提取了四个因素（开放性、友善性、粗暴性和神经质）。我们检查了微卫星基因型、年龄、性别、绝育状态和性格评分之间的关联。与同类相比，年轻的猫在开放性方面得分更高，公猫在友善度方面得分更高，母猫和绝育猫在粗糙度方面得分更高。当我们根据等位基因的长度将样本分为三组时，我们发现友善度和 MS3 之间存在略微显着的关联。此外，我们发现 MS4 中的基因型对友善度存在性别介导的影响，从而对女性和男性产生不同的影响。我们的发现表明，杂种猫的 MS3 和 MS4 等位基因比纯种猫更长，而且这些猫在友善度方面得分更高，这支持了之前的发现。然而，未来的研究（例如对具有明显不同起源或个性的纯种猫进行比较）需要确定 OXTR 中的遗传变异与个性的关联。
A growing number of studies have explored the oxytocin system in humans and non-human animals, and some have found important genetic polymorphisms in the oxytocin receptor gene (OXTR) associated with the bonding system, social behaviors, and personality in several species. Although single nucleotide polymorphisms in OXTR have been well-examined in various species, microsatellites (or short tandem repeats) adjacent to OXTR have rarely been studied, despite some suggestions that microsatellite polymorphisms near genes might play a role in genetic transcription and translation. In this study, we surveyed microsatellites in the upstream, intron, and downstream regions of OXTR in domestic cats (Felis catus). We succeeded in amplifying 5 out of 10 regions, and recognized these five regions as polymorphic. We compared allele frequencies in these five regions between mongrel cats in Japan (n = 100) and cats of 10 pure breeds (n = 40). There were significant differences in allele frequencies between the two populations in all microsatellite regions. Additionally, the owners of mongrel cats answered a comprehensive personality questionnaire, and factor analysis extracted four factors (Openness, Friendliness, Roughness, and Neuroticism). We examined the association between the microsatellite genotypes, age, sex, neutering status, and personality scores. Compared to their counterparts, younger cats tended to score higher on Openness, male cats scored higher on Friendliness, and female and neutered cats scored higher on Roughness. When we divided the sample into three groups depending on the length of alleles, we found a marginally significant association between Friendliness and MS3. Additionally, we found a sex-mediated effect of genotypes in MS4 on Friendliness, resulting in different effects on females and males. Our findings that mongrel cats had longer alleles in MS3 and MS4 than purebred cats, and that those cats tended to score higher on Friendliness, supported the previous findings. However, future studies such as comparison between purebred cats with apparently different origin or personality are required to determine the association of genetic variants in the OXTR with personality.
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影响因子：
1.9
作者：
Martins WS;Lucas DC;Neves KF;Bertioli DJ
通讯作者：
Bertioli DJ
影响因子：
5.7
作者：
Chen, Frances S.;Johnson, Susan C.
通讯作者：
Johnson, Susan C.
影响因子：
3.7
作者：
Sawaya S;Bagshaw A;Buschiazzo E;Kumar P;Chowdhury S;Black MA;Gemmell N
通讯作者：
Gemmell N
影响因子：
3.8
作者：
Shang S;Wu N;Su Y
通讯作者：
Su Y
DOI：
10.1073/pnas.0909579106
发表时间：
2009-12-15
影响因子：
11.1
作者：
Rodrigues, Sarina M.;Saslow, Laura R.;Keltner, Dacher
通讯作者：
Keltner, Dacher