Effect of temperature on FAD and NADH-derived signals and neurometabolic coupling in the mouse auditory and motor cortex.
Effect of temperature on FAD and NADH-derived signals and neurometabolic coupling in the mouse auditory and motor cortex.
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DOI：
10.1007/s00424-017-2037-4
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Llano DA
中科院分区：
文献类型：
--
作者：
Ibrahim BA;Wang H;Lesicko AMH;Bucci B;Paul K;Llano DA
神经元代谢与突触活动的紧密耦合对于确保代谢底物的供应满足神经元信号传导的需求至关重要。考虑到温度对代谢的影响，以及临床低温期间观察到的脑温度的大幅波动，我们研究了温度对神经代谢偶联的影响。测量氧化形式的黄素腺嘌呤二核苷酸（FAD）和还原形式的烟酰胺腺嘌呤二核苷酸（NADH）的固有荧光信号及其比率以评估神经代谢状态，并记录局部场电位以测量小鼠脑中的突触活性。脑切片制备用于消除血流的潜在影响。在低于29°C的温度范围内观察到代谢信号和局部场电位振幅之间的紧密耦合。然而，在29 °C以上，代谢和突触特征发散，使得FAD信号减弱，但局部场电位保持其幅度。还观察到，在高温下观察到的FAD信号的下降（以及因此突触和代谢事件之间的解耦）是由高温下的低FAD可用性驱动的。这些数据表明，神经代谢耦合，被认为是至关重要的，以确保大脑的代谢健康，可能会显示温度依赖性，并与温度依赖性变化的FAD供应。
Tight coupling of neuronal metabolism to synaptic activity is critical to ensure that the supply of metabolic substrates meets the demands of neuronal signaling. Given the impact of temperature on metabolism, and the wide fluctuations of brain temperature observed during clinical hypothermia, we examined the effect of temperature on neurometabolic coupling. Intrinsic fluorescence signals of the oxidized form of flavin adenine dinucleotide (FAD) and the reduced form of nicotinamide adenine dinucleotide (NADH), and their ratios, were measured to assess neural metabolic state and local field potentials were recorded to measure synaptic activity in the mouse brain. Brain slice preparations were used to remove the potential impacts of blood flow. Tight coupling between metabolic signals and local field potential amplitudes was observed at a range of temperatures below 29°C. However, above 29 °C, the metabolic and synaptic signatures diverged such that FAD signals were diminished, but local field potentials retained their amplitude. It was also observed that the declines in the FAD signals seen at high temperatures (and hence the decoupling between synaptic and metabolic events), is driven by low FAD availability at high temperatures. These data suggest that neurometabolic coupling, thought to be critical for ensuring the metabolic health of the brain, may show temperature dependence, and is related to temperature-dependent changes in FAD supplies.
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DOI：
10.1093/cercor/bht332
发表时间：
2015-06
期刊：
Cerebral cortex (New York, N.Y. : 1991)
影响因子：
--
作者：
Cooper JM;Gadian DG;Jentschke S;Goldman A;Munoz M;Pitts G;Banks T;Chong WK;Hoskote A;Deanfield J;Baldeweg T;de Haan M;Mishkin M;Vargha-Khadem F
通讯作者：
Vargha-Khadem F
影响因子：
168.9
作者：
Gluckman, PD;Wyatt, JS;Gunn, AJ
通讯作者：
Gunn, AJ
影响因子：
3
作者：
Hajos, Norbert;Mody, Istvan
通讯作者：
Mody, Istvan
影响因子：
4.5
作者：
Gerich, Florian J.;Funke, Frank;Hildebrandt, Belinda;Fasshauer, Martin;Mueller, Michael
通讯作者：
Mueller, Michael
影响因子：
158.5
作者：
Bernard, SA;Gray, TW;Smith, K
通讯作者：
Smith, K