Protease inhibitor levels in periodontal health and disease.
Protease inhibitor levels in periodontal health and disease.
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DOI：
10.1111/j.1600-0765.2011.01425.x
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发表时间：
2012-04
影响因子：
3.5
通讯作者：
Chung WO
中科院分区：
文献类型：
--
作者：
Kretschmar S;Yin L;Roberts F;London R;Flemmig TT;Arushanov D;Kaiyala K;Chung WO
我们之前的研究表明，在培养的牙龈上皮细胞中，蛋白酶抑制剂被周围病原体牙龈卟啉单胞菌减弱。我们假设在牙龈卟啉单胞菌水平可能较高的较晚期牙周部位中存在较少的蛋白酶抑制剂。本研究的目的是探讨龈沟液（GCF）中蛋白酶抑制剂（SLPI、ELAFIN、SCCA）水平与龈下菌斑中牙龈卟啉单胞菌数量之间的关系。使用来自未患牙周炎 (n=18) 和患有中度至晚期牙周炎 (n=41) 的受试者的牙菌斑样本，通过实时 PCR 来量化牙龈卟啉单胞菌。通过 ELISA 测定所有受试者 GCF 中的蛋白酶抑制剂水平。 68.3% 的牙周炎患者检测到牙龈卟啉单胞菌，而16.7% 的无牙周炎患者检测到牙龈卟啉单胞菌。与没有牙周炎的对照受试者相比，患有牙周炎和牙菌斑中牙龈卟啉单胞菌的受试者表现出较低的 SLPI 和 ELAFIN 水平 (p<0.001)。牙周病患者 GCF 中的 SLPI 也降低（p<0.05），但未检测到牙龈卟啉单胞菌水平。牙龈卟啉单胞菌水平高与低的牙周患者表现出 SLPI 和 ELAFIN 浓度平均水平的倒数。 SLPI 和 ELAFIN 浓度降低可能导致宿主保护能力丧失，并增加慢性感染导致的崩溃的可能性。这项研究的工作可能有助于寻找牙周健康和疾病的诊断和预后标志物，并可能进一步帮助寻找针对牙周炎症的药理学靶点。
Our previous study showed protease inhibitors were attenuated by periopathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize fewer protease inhibitors would be present in more advanced periodontal sites where the level of P. gingivalis may be high. The goal of this study was to investigate the relationship between the protease inhibitor (SLPI, ELAFIN, SCCA) levels in gingival crevicular fluid (GCF) and the number of P. gingivalis in subgingival plaque. Plaque samples from subjects without (n=18) and with moderate to advanced periodontitis (n=41) were used to quantify P. gingivalis using real-time PCR. Protease inhibitor levels in the GCF of all the subjects were determined by ELISA. P. gingivalis was detected in 68.3% of subjects with periodontitis, while 16.7% of patients without periodontitis had detectable level of P. gingivalis. Subjects with periodontitis and P. gingivalis in their plaque exhibited lower SLPI and ELAFIN levels (p<0.001) compare to control subjects without periodontitis. SLPI was also reduced (p<0.05) in GCF of periodontal patients without detectable level of P. gingivalis. Periodontal patients with high vs. low levels of P. gingivalis exhibited reciprocal mean levels of SLPI and ELAFIN concentrations. The reduced concentrations of SLPI and ELAFIN may contribute to the loss of host protective capacity and increase susceptibility to breakdown from chronic infection. The work of this investigation may aid in finding diagnostic and prognostic markers in periodontal health and disease and may further help in finding pharmacological targets directed against periodontal inflammation.
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影响因子：
3.7
作者：
Kantyka T;Latendorf T;Wiedow O;Bartels J;Gläser R;Dubin G;Schröder JM;Potempa J;Meyer-Hoffert U
通讯作者：
Meyer-Hoffert U
影响因子：
3.5
作者：
Cox, S. W.;Rodriguez-Gonzalez, E. M.;Eley, B. M.
通讯作者：
Eley, B. M.
影响因子：
4.8
作者：
Guyot, Nicolas;Butler, Marcus W.;McElvaney, Noel G.
通讯作者：
McElvaney, Noel G.
影响因子：
3
作者：
GIANNOPOULOU, C;DEMEURISSE, C;CIMASONI, G
通讯作者：
CIMASONI, G
影响因子：
3
作者：
GIANNOPOULOU, C;DEFELICE, R;CIMASONI, G
通讯作者：
CIMASONI, G