Dietary exposure of 17-alpha ethinylestradiol modulates physiological endpoints and gene signaling pathways in female largemouth bass (Micropterus salmoides).
Dietary exposure of 17-alpha ethinylestradiol modulates physiological endpoints and gene signaling pathways in female largemouth bass (Micropterus salmoides).
复制标题
DOI：
10.1016/j.aquatox.2014.08.008
复制
发表时间：
2014-11
影响因子：
4.5
通讯作者：
Denslow, Nancy D.
中科院分区：
文献类型：
--
作者：
Colli-Dula, Reyna-Cristina;Martyniuk, Christopher J.;Kroll, Kevin J.;Prucha, Melinda S.;Kozuch, Marianne;Barber, David S.;Denslow, Nancy D.
17 α-炔雌醇（EE 2），用于人类生育控制，是一种有效的雌激素，在废水中发现低浓度（ng/L）。EE 2能够干扰鱼类的内分泌系统，影响生殖，从而导致种群水平的影响。本研究的目的是确定EE 2的饮食暴露是否会改变肝脏和卵巢中的基因表达模式和关键途径，以及这些是否可能与雌性大口黑鲈卵子发育期间的生殖终点相关。雌性LMB接受70 ng EE 2/g饲料（以体重的1%给予饲料），持续60天。EE 2饮食暴露显着降低血浆卵黄蛋白原浓度的70%。肝指数和性腺指数也分别下降了38.5%和40%。转录组学分析显示，与卵巢相比，肝脏中稳态mRNA水平的变化更多。与生殖相关的基因差异表达，如卵黄蛋白原在肝脏和芳香化酶在性腺。此外，一组与氧化应激相关的基因（例如谷胱甘肽还原酶和谷胱甘肽过氧化物酶）在肝脏中被鉴定为改变，与免疫系统相关的基因（例如补体成分1和巨噬细胞诱导的C型凝集素）在性腺中被改变。在使用0.2 ng EE 2/g饲料进行60天的后续研究中，观察到类似的表型和基因表达变化，支持较高浓度下的这些发现。这项研究提供了新的见解，如何饮食暴露于EE 2干扰内分泌信号通路的女性LMB在生殖卵子发生的关键时期。
17alpha-ethinylestradiol (EE2), used for birth control in humans, is a potent estrogen that is found in wastewater at low concentrations (ng/L). EE2 has the ability to interfere with the endocrine system of fish, affecting reproduction which can result in population level effects. The objective of this study was to determine if dietary exposure to EE2 would alter gene expression patterns and key pathways in the liver and ovary and whether these could be associated with reproductive endpoints in female largemouth bass during egg development. Female LMB received 70 ng EE2/g feed (feed administered at 1% of body weight) for 60 days. EE2 dietary exposure significantly reduced plasma vitellogenin concentrations by 70%. Hepatosomatic and gonadosomatic indices were also decreased with EE2 feeding by 38.5% and 40%, respectively. Transcriptomic profiling revealed that there were more changes in steady state mRNA levels in the liver compared to the ovary. Genes associated with reproduction were differentially expressed such as vitellogenin in the liver and aromatase in the gonad. In addition, a set of genes related with oxidative stress (e.g. glutathione reductase and glutathione peroxidase) were identified as altered in the liver and genes associated with the immune system (e.g. complement component 1, and macrophage-inducible C-type lectin) were altered in the gonad. In a follow-up study with 0.2 ng EE2/g feed for 60 days, similar phenotypic and gene expression changes were observed that support these findings with the higher concentrations. This study provides new insights into how dietary exposure to EE2 interferes with endocrine signaling pathways in female LMB during a critical period of reproductive oogenesis.
登录
查看更多内容
影响因子：
4.5
作者：
Ankley, Gerald T.;Bencic, David C.;Watanabe, Karen H.
通讯作者：
Watanabe, Karen H.
影响因子：
5.8
作者：
Casanova-Nakayama, Ayako;Wenger, Michael;Segner, Helmut
通讯作者：
Segner, Helmut
影响因子：
4.3
作者：
Chikae, M;Ikeda, R;Tamiya, E
通讯作者：
Tamiya, E
影响因子：
3.8
作者：
Ankley, GT;Kahl, MD;Leino, RL
通讯作者：
Leino, RL
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y