Reconstitution of mammary epithelial morphogenesis by murine embryonic stem cells undergoing hematopoietic stem cell differentiation.
Reconstitution of mammary epithelial morphogenesis by murine embryonic stem cells undergoing hematopoietic stem cell differentiation.
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鼠类胚胎干细胞经历造血干细胞分化的乳腺上皮形态发生。
DOI：
10.1371/journal.pone.0009707
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发表时间：
2010-03-15
期刊：
影响因子：
3.7
通讯作者：
Avraham, Hava Karsenty
中科院分区：
文献类型：
--
作者：
Jiang, Shuxian;Lee, Byeong-Chel;Fu, Yigong;Avraham, Shalom;Lim, Bing;Avraham, Hava Karsenty
Mammary stem cells are maintained within specific microenvironments and recruited throughout lifetime to reconstitute de novo the mammary gland. Mammary stem cells have been isolated through the identification of specific cell surface markers and in vivo transplantation into cleared mammary fat pads. Accumulating evidence showed that during the reformation of mammary stem cell niches by dispersed epithelial cells in the context of the intact epithelium-free mammary stroma, non-mammary epithelial cells may be sequestered and reprogrammed to perform mammary epithelial cell functions and to adopt mammary epithelial characteristics during reconstruction of mammary epithelium in regenerating mammary tissue in vivo. To examine whether other types of progenitor cells are able to contribute to mammary branching morphogenesis, we examined the potential of murine embryonic stem (mES) cells, undergoing hematopoietic differentiation, to support mammary reconstitution in vivo. We observed that cells from day 14 embryoid bodies (EBs) under hematopoietic differentiation condition, but not supernatants derived from these cells, when transplanted into denuded mammary fat pads, were able to contribute to both the luminal and myoepithelial lineages in branching ductal structures resembling the ductal-alveolar architecture of the mammary tree. No teratomas were observed when these cells were transplanted in vivo. Our data provide evidence for the dominance of the tissue-specific mammary stem cell niche and its role in directing mES cells, undergoing hematopoietic differentiation, to reprogram into mammary epithelial cells and to promote mammary epithelial morphogenesis. These studies should also provide insights into regeneration of damaged mammary gland and the role of the mammary microenvironment in reprogramming cell fate.
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影响因子：
3.8
作者：
Smith, GH
通讯作者：
Smith, GH
影响因子：
3.7
作者：
DANIEL, CW;YOUNG, LJT
通讯作者：
YOUNG, LJT
影响因子：
3.7
作者：
Jiang S;Fu Y;Williams J;Wood J;Pandarinathan L;Avraham S;Makriyannis A;Avraham S;Avraham HK
通讯作者：
Avraham HK
影响因子：
56.9
作者：
DANIEL, CW;DEOME, KB
通讯作者：
DEOME, KB
DOI：
10.4161/cc.8.8.8189
发表时间：
2009-04-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Boulanger CA;Smith GH
通讯作者：
Smith GH