A novel caspase-1 mediates inflammatory responses and pyroptosis in sea cucumber Apostichopus japonicus
A novel caspase-1 mediates inflammatory responses and pyroptosis in sea cucumber Apostichopus japonicus
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新型 caspase-1 介导刺参炎症反应和细胞焦亡
DOI：
10.1016/j.aquaculture.2019.734399
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发表时间：
2019-11
期刊：
影响因子：
4.5
通讯作者：
Guo Ming
中科院分区：
文献类型：
--
作者：
Shao Yina;Che Zhongjie;Li Chenghua;Zhang Weiwei;Zhao Xuelin;Guo Ming
Caspase-1 is the most important inflammatory caspase and plays necessary roles in cell death. In the current study, a novel caspase-1 homolog was identified from sea cucumber Apostichopus japonicus; this homolog encoded a predicted protein containing a CASc domain with the pentapetide active site173QACRG177.Ajcaspase-1mRNA was ubiquitously expressed in all detected tissues, with higher levels found in respiratory trees and coelomocytes. Subcellular localization of Ajcaspase-1 mainly occurred in the nucleus. The transcript expression ofAjcaspase-1was remarkably up-regulated in coelomocytes after Vibrio splendidus challenge in vivo and LPS stimulation in vitro. Recombinant Ajcaspase-1 (rAjcaspase-1) and the C-terminal of Ajcaspase-1 (rAjcaspase-1-C) revealed high levels of LPS binding activity and exhibited proteolytic activities toward the substrates Ac-YVAD-pNA and Ac-DEVD-pNA. The high activity of these enzymes in Ac-YVAD-pNA suggests that Ajcaspase-1 shares common caspase-1 activity. No obvious activities were detected for rAjcaspase-1-N. More importantly, rAjcaspase-1 significantly increased the expressions ofHomoIL-1βandHomoGSDMDmRNA in HEK293 cells. Our results clearly indicate that Ajcaspase-1 may participate in the pyroptosis pathway by regulating inflammatory responses.
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影响因子：
29.7
作者：
Davis BK;Wen H;Ting JP
通讯作者：
Ting JP
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
通讯作者：
--
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影响因子：
4.7
作者：
Qu Chen;Yang Wen;Xu Qingsong;Sun Jiejie;Lu Mengmeng;Wang Ying;Liu Chao;Wang Weilin;Wang Lingling;Song Linsheng
通讯作者：
Song Linsheng
影响因子：
7.2
作者：
McIlwain, David R.;Berger, Thorsten;Mak, Tak W.
通讯作者：
Mak, Tak W.
影响因子：
4.7
作者：
R. Wen;Fuhua Li;Zheng Sun;Shihao Li;J. Xiang
通讯作者：
R. Wen;Fuhua Li;Zheng Sun;Shihao Li;J. Xiang