Closely related Lak megaphages replicate in the microbiomes of diverse animals.
Closely related Lak megaphages replicate in the microbiomes of diverse animals.
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DOI：
10.1016/j.isci.2021.102875
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发表时间：
2021-08-20
期刊：
影响因子：
5.8
通讯作者：
Santini JM
中科院分区：
文献类型：
--
作者：
Crisci MA;Chen LX;Devoto AE;Borges AL;Bordin N;Sachdeva R;Tett A;Sharrar AM;Segata N;Debenedetti F;Bailey M;Burt R;Wood RM;Rowden LJ;Corsini PM;van Winden S;Holmes MA;Lei S;Banfield JF;Santini JM
最近有报道称，具有替代编码的约540 kbp基因组的Lak peptide在食用非西方饮食的人类、狒狒和猪的微生物组中的普雷沃氏菌中复制。在这里，我们探索Lak噬菌体的多样性和更广泛的分布使用诊断聚合酶链反应和基因组解析宏基因组学。在包括爬行动物在内的13种动物中检测到了Lak，并且在猪中特别普遍。通过猪胃肠道追踪Lak发现，与前肠相比，后肠中Lak显著富集。我们重建了34个新的Lak基因组，包括6个策划的完整基因组，所有这些都是交替编码的。为来自马微生物组的最深分支的Lak重建的非常大的（约660 kbp）完整基因组也被交替编码。从Lak基因组中，我们确定了与特定动物物种相关的蛋白质;值得注意的是，大多数蛋白质没有功能预测。在不同的动物中存在密切相关的Lak，表明与宿主特异性适应相结合的简单分布。PCR和基因组解析宏基因组学揭示了13种动物中的Lak蛋白Lak在猪后肠中含量丰富，与生长性能具有潜在的相关性34个新的Lak噬菌体基因组呈现出扩大的大小范围和遗传性来自特定微生物组的独特Lak蛋白家族表明动物宿主适应性病毒学;微生物组学;组学
Lak phages with alternatively coded ∼540 kbp genomes were recently reported to replicate in Prevotella in microbiomes of humans that consume a non-Western diet, baboons, and pigs. Here, we explore Lak phage diversity and broader distribution using diagnostic polymerase chain reaction and genome-resolved metagenomics. Lak phages were detected in 13 animal types, including reptiles, and are particularly prevalent in pigs. Tracking Lak through the pig gastrointestinal tract revealed significant enrichment in the hindgut compared to the foregut. We reconstructed 34 new Lak genomes, including six curated complete genomes, all of which are alternatively coded. An anomalously large (∼660 kbp) complete genome reconstructed for the most deeply branched Lak from a horse microbiome is also alternatively coded. From the Lak genomes, we identified proteins associated with specific animal species; notably, most have no functional predictions. The presence of closely related Lak phages in diverse animals indicates facile distribution coupled to host-specific adaptation. PCR and genome-resolved metagenomics revealed Lak phages in 13 animal species Lak is abundant in the pig hindgut, with potential relevance to growth performance Thirty-four new Lak phage genomes present expanded size range and phylogeny Unique Lak protein families from specific microbiomes suggest animal host adaptation Virology; Microbiome; Omics
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
DOI：
10.1073/pnas.1508355112
发表时间：
2015-11-03
影响因子：
11.1
作者：
Barr, Jeremy J.;Auro, Rita;Rohwer, Forest
通讯作者：
Rohwer, Forest
影响因子：
15.5
作者：
Gorvitovskaia A;Holmes SP;Huse SM
通讯作者：
Huse SM
影响因子：
56.9
作者：
Ivanova, Natalia N.;Schwientek, Patrick;Rubin, Edward M.
通讯作者：
Rubin, Edward M.
DOI：
10.1073/pnas.1305923110
发表时间：
2013-06-25
影响因子：
11.1
作者：
Barr, Jeremy J.;Auro, Rita;Rohwer, Forest
通讯作者：
Rohwer, Forest