The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci.
The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci.
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DOI：
10.1007/s00122-016-2796-x
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发表时间：
2017-01
影响因子：
5.4
通讯作者：
Nicholson, P.
中科院分区：
文献类型：
--
作者：
Pasquariello, M.;Ham, J.;Burt, C.;Jahier, J.;Paillard, S.;Uauy, C.;Nicholson, P.
表型分析和作图数据表明，7D和7A上分别含有抗纹枯病基因Pch1和Pch2的染色体区间不重叠，因此这些基因不是同源基因。纹枯病是一种生长在温带地区的禾谷类作物的茎基真菌病害。目前有两种主要的抗性可用于小麦。Pch1基因定位于7D染色体远端，是一个从室内山羊草(Aegilopventricosa)转入小麦的有效的主效基因。Pch2是来自Cappelle Desprez的一个中等抗性基因，位于7AL末端。Pch1和Pch2分别在7D和7A上的相对位置表明它们是同源的。利用单粒种子得体的重组F7群体对Pch2在7A上的位置进行了精炼。设计为7D的新标记还允许进一步定义Pch1的位置。我们利用小麦与远端短柄麦的共生关系，开发了标记品种间和种间SNPs的7A和7D特异KASP标记，并比较了Pch1和Pch2在7D和7A上的相对位置。总而言之，表型和图谱数据显示，包含Pch1和Pch2的间隔不重叠，因此，它们不能被认为是同源基因。利用这些信息，我们分析了两个在7A上携带Pch1的硬粒小麦品系，以确定Ae.ventricosa导入是否延伸到与Pch2相关的区域。这证实了7A上的导入位于Pch2的远端，这进一步证明了这些基因不是同源基因。然而，利用该材料在四倍体背景下对7A上的Pch1和Pch2进行金字塔聚合，以及在六倍体背景下增加Pch1和Pch2的拷贝数也是可行的。
Phenotyping and mapping data reveal that chromosome intervals containing eyespot resistance genes Pch1 and Pch2 on 7D and 7A, respectively, do not overlap, and thus, these genes are not homoeloci. Eyespot is a stem-base fungal disease of cereals growing in temperate regions. Two main resistances are currently available for use in wheat. Pch1 is a potent single major gene transferred to wheat from Aegilops ventricosa and located on the distal end of chromosome 7D. Pch2, a moderate resistance deriving from Cappelle Desprez, is located at the end of 7AL. The relative positions of Pch1 and Pch2 on 7D and 7A, respectively, suggest that they are homoeoloci. A single seed decent recombinant F7 population was used to refine the position of Pch2 on 7A. New markers designed to 7D also allowed the position of Pch1 to be further defined. We exploited the syntenic relationship between Brachypodium distachyon and wheat to develop 7A and 7D specific KASP markers tagging inter-varietal and interspecific SNPs and allow the comparison of the relative positions of Pch1 and Pch2 on 7D and 7A. Together, phenotyping and mapping data reveal that the intervals containing Pch1 and Pch2 do not overlap, and thus, they cannot be considered homoeloci. Using this information, we analysed two durum wheat lines carrying Pch1 on 7A to determine whether the Ae.ventricosa introgression extended into the region associated with Pch2. This identified that the introgression is distal to Pch2 on 7A, providing further evidence that the genes are not homoeoloci. However, it is feasible to use this material to pyramid Pch1 and Pch2 on 7A in a tetraploid background and also to increase the copy number of Pch1 in combination with Pch2 in a hexaploid background.
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影响因子：
5.4
作者：
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通讯作者：
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通讯作者：
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