Hematopoietic stem cells contribute to lymphatic endothelium.
Hematopoietic stem cells contribute to lymphatic endothelium.
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DOI：
10.1371/journal.pone.0003812
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Fleming WH
中科院分区：
文献类型：
--
作者：
Jiang S;Bailey AS;Goldman DC;Swain JR;Wong MH;Streeter PR;Fleming WH
尽管淋巴系统在胚胎中作为静脉血管的延伸而出现，但人们对循环祖细胞在淋巴内皮的维持或发育中的作用知之甚少。在这里，我们研究了造血干细胞（hsc）是否有可能产生淋巴内皮细胞（LEC）。将标记的造血干细胞转移到辐照受体后，在几个组织中发现了共同表达淋巴内皮标志物Lyve-1和VEGFR-3的供体来源LEC。hsc来源的LEC持续存在超过12个月，并贡献了约3-4%的淋巴管。在移植了普通髓系祖细胞和粒细胞/巨噬细胞祖细胞的小鼠中未检测到供体来源的LECs，这表明髓系谱系承诺不是HSC对淋巴内皮贡献的必要步骤。对异种共生小鼠的分析揭示了在没有急性损伤的情况下存在功能性循环淋巴祖细胞的直接证据。此外，将造血干细胞移植到ApcMin/+小鼠体内，导致供体来源的LEC结合到自发产生的肠道肿瘤的淋巴管中。我们的研究结果表明造血干细胞可以促进正常和肿瘤相关的淋巴内皮。这些发现表明，造血干细胞的修饰可能是靶向肿瘤转移和减轻淋巴系统疾病的新途径。
Although the lymphatic system arises as an extension of venous vessels in the embryo, little is known about the role of circulating progenitors in the maintenance or development of lymphatic endothelium. Here, we investigated whether hematopoietic stem cells (HSCs) have the potential to give rise to lymphatic endothelial cells (LEC). Following the transfer of marked HSCs into irradiated recipients, donor-derived LEC that co-express the lymphatic endothelial markers Lyve-1 and VEGFR-3 were identified in several tissues. HSC-derived LEC persisted for more than 12 months and contributed to ∼3–4% of lymphatic vessels. Donor-derived LECs were not detected in mice transplanted with common myeloid progenitors and granulocyte/macrophage progenitors, suggesting that myeloid lineage commitment is not a requisite step in HSC contribution to lymphatic endothelium. Analysis of parabiotic mice revealed direct evidence for the existence of functional, circulating lymphatic progenitors in the absence of acute injury. Furthermore, the transplantation of HSCs into ApcMin/+ mice resulted in the incorporation of donor-derived LEC into the lymphatic vessels of spontaneously arising intestinal tumors. Our results indicate that HSCs can contribute to normal and tumor associated lymphatic endothelium. These findings suggest that the modification of HSCs may be a novel approach for targeting tumor metastasis and attenuating diseases of the lymphatic system.
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影响因子：
15.9
作者：
Maruyama, K;Li, M;Streilein, JW
通讯作者：
Streilein, JW
影响因子：
7.8
作者：
Banerji, S;Ni, J;Wang, S X;Clasper, S;Su, J;Tammi, R;Jones, M;Jackson, D G
通讯作者：
Jackson, D G
DOI：
10.1073/pnas.0604203103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Bailey, Alexis S.;Willenbring, Holger;Fleming, William H.
通讯作者：
Fleming, William H.
影响因子：
11.2
作者：
He, YL;Rajantie, I;Alitalo, K
通讯作者：
Alitalo, K
影响因子：
4.4
作者：
Larrivée, B;Niessen, K;Karsan, A
通讯作者：
Karsan, A