Mosquito cryptochromes expressed in Drosophila confer species-specific behavioral light responses.
Mosquito cryptochromes expressed in Drosophila confer species-specific behavioral light responses.
复制标题
DOI：
10.1016/j.cub.2022.07.021
复制
发表时间：
2022-09-12
期刊：
影响因子：
9.2
通讯作者：
Holmes, Todd C.
中科院分区：
文献类型：
--
作者：
Au, David D.;Foden, Alexander J.;Park, Soo Jee;Nguyen, Thanh H.;Liu, Jenny C.;Tran, Mary D.;Jaime, Olga G.;Yu, Zhaoxia;Holmes, Todd C.
CRYPTOCHROME （CRY）是一种短波长的光敏光感受器，在果蝇的昼夜节律神经元和眼睛中表达，调节光诱发的昼夜节律钟重置。强烈地，光唤起昼夜节律神经元腹侧亚群的快速电兴奋，并赋予昼夜节律调制的对短波长的回避行为反应。最近的研究表明，白天活跃的蚊子和夜间活跃的蚊子对短波长的光的回避和吸引行为反应有很大不同；这些行为反应被蚊子持续暴露在光线下的CRY蛋白降解所减弱。为了确定CRY1s是否介导了行为和电生理光反应的物种特异性编码，我们使用了“空神经元”方法，在没有哭泣的果蝇背景下转基因表达了白天的埃及伊蚊（AeCRY1）和夜间的冈比亚按蚊（AgCRY1）。AeCRY1的光敏性远低于AgCRY1或DmCRY，这可以从持续光照后的部分行为节律中看出。值得注意的是，夜间AgCRY1的表达在恒定白光下的存活率较低，AeCRY1的表达在较小程度上也是如此。相对于AeCRY1， AgCRY1介导的365 nm紫外光的电生理细胞自主反应明显更强。AgCRY1表达介导对635 nm红光的电生理和行为敏感性，而AeCRY1不表达，这与蚊种特异性红光反应一致。AgCRY1和DmCRY介导不同光强阈值下对紫外光的强度依赖性回避行为，而AeCRY1则没有，从而模仿蚊子和苍蝇的行为。这些发现强调了CRY作为一种关键的非图像形成视觉光感受器，以特定物种的方式介导生理和行为光反应。Au等研究表明，在果蝇中转基因表达昼行性和夜行性蚊子的CRY，可在蚊子中产生与其功能相匹配的光诱发行为和神经生理效应。夜蚊CRY1表现出明显高于昼蚊CRY1的行为和电生理光反应。
CRYPTOCHROME (CRY) is a short-wavelength light-sensitive photoreceptor expressed in a subset of circadian neurons and eyes in Drosophila that regulates light evoked circadian clock resetting. Acutely, light evokes rapid electrical excitation of the ventral lateral subset of circadian neurons and confers circadian modulated avoidance behavioral responses to short wavelength light. Recent work shows dramatically different avoidance versus attraction behavioral responses to short wavelength light in day-active versus night-active mosquitoes; and that these behavioral responses are attenuated by CRY protein degradation by constant light exposure in mosquitoes. To determine whether CRY1s mediate species-specific coding for behavioral and electrophysiological light-responses, we used an “empty neuron” approach and transgenically expressed diurnal Aedes aegypti (AeCRY1) versus nocturnal Anopheles gambiae (AgCRY1) in a cry-null Drosophila background. AeCRY1 is much less light sensitive than either AgCRY1 or DmCRY as shown by partial behavioral rhythmicity following constant light exposure. Remarkably, expression of nocturnal AgCRY1 confers low survival to constant white light as does expression of AeCRY1 to a lesser extent. AgCRY1 mediates significantly stronger electrophysiological cell autonomous responses to 365 nm ultraviolet (UV) light relative to AeCRY1. AgCRY1 expression mediates electrophysiological and behavioral sensitivity to 635 nm red light while AeCRY1 does not, consistent with species-specific mosquito red light responses. AgCRY1 and DmCRY mediate intensity-dependent avoidance behavior to UV light at different light intensity thresholds, while AeCRY1 does not, thus mimicking mosquito and fly behaviors. These findings highlight CRY as a key non-image forming visual photoreceptor that mediates physiological and behavioral light-responses in a species-specific fashion. Au et al. show that transgenic expression of diurnal and nocturnal mosquito species’ CRY in Drosophila transduce light-evoked behavior and neurophysiological effects matching their functions in mosquitoes. Nocturnal mosquito CRY1 shows significantly greater behavioral and electrophysiological light responses than diurnal mosquito CRY1.
登录
查看更多内容
影响因子：
9.2
作者：
Baik, Lisa S.;Nave, Ceazar;Holmes, Todd C.
通讯作者：
Holmes, Todd C.
影响因子：
16.2
作者：
Dobritsa, AA;van der Goes van Naters, W;Carlson, JR
通讯作者：
Carlson, JR
DOI：
10.1007/s003590050192
发表时间：
1998-04-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
影响因子：
--
作者：
Helfrich-Förster, C
通讯作者：
Helfrich-Förster, C
影响因子：
4.8
作者：
Berndt, Alex;Kottke, Tilman;Wolf, Eva
通讯作者：
Wolf, Eva
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y