Prenatal opioid administration induces shared alterations to the maternal and offspring gut microbiome: A preliminary analysis.
Prenatal opioid administration induces shared alterations to the maternal and offspring gut microbiome: A preliminary analysis.
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DOI：
10.1016/j.drugalcdep.2021.108914
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发表时间：
2021-10-01
影响因子：
4.2
通讯作者：
Atwood BK
中科院分区：
文献类型：
--
作者：
Grecco GG;Gao Y;Gao H;Liu Y;Atwood BK
虽然许多研究已经描述了产前阿片类药物暴露对发育的影响，但阿片类药物如何发挥发育障碍的可能机制仍然难以捉摸。新出现的证据表明，母体肠道微生物组的破坏可能会改变后代的发育;然而，迄今为止，还没有研究考察母体阿片类药物治疗对母体-后代微生物组生态失调的影响。采用产前美沙酮暴露（PME）的小鼠模型来评估母体阿片类药物治疗对美沙酮治疗的母体（MD）及其后代的微生物组的影响。从母鼠（每个处理组n=8）采集粪便样品，每个母鼠1只雄性和1只雌性后代（每个处理组每个性别n=8只后代）用于16 s rRNA测序。美沙酮治疗显着增加了微生物多样性，并导致家庭水平的细菌丰度扩大。相关性分析显示，显着的正相关关系之间的大坝和后代的多样性措施，表明美沙酮诱导的变化，在微生物群落之间的大坝和后代共享。处理组之间母体中的16个特征和后代中的10个特征显著差异丰富，其中许多特征对应于毛螺菌科NK 4A 136属。在MD和PME后代中被鉴定为差异丰富的六个特征中，所有特征都被分配到毛螺菌科NK 4A 136组，并且丰度在母体和后代之间表现出强的正相关性。这些初步研究结果表明，妊娠期间母体阿片类药物治疗改变了母体微生物组的组成，并且在后代中也类似地观察到这种阿片类药物诱导的变化，这可能导致先前描述的发育表型受损。
While many studies have described the impact of prenatal opioid exposure on development, possible mechanisms for how opioids exert developmental impairments remain elusive. Emerging evidence indicates disruptions in the maternal gut microbiome can alter offspring development; however, no studies to date have examined the impact of maternal opioid treatment on maternal-offspring microbiome dysbiosis. A mouse model of prenatal methadone exposure (PME) was employed to assess the impact of maternal opioid treatment on the microbiome of methadone-treated dams (MD) and their offspring. Fecal samples were collected from dams (n=8 per treatment), one male and one female offspring per dam (n=8 offspring per sex per treatment) for 16s rRNA sequencing. Methadone treatment significantly increased the microbial diversity and led to an expansion in family level bacterial abundance. Correlational analysis revealed significant positive associations between dam and offspring measures of diversity indicating methadone-induced shifts in the microbial communities are shared between dam and offspring. Sixteen features in dams and 10 features in offspring were significantly differentially abundant between treatment groups with many features corresponding to the Lachnospiraceae NK4A136 genus. Of the six features identified as differentially abundant in both MD and PME offspring, all were assigned to the Lachnospiraceae NK4A136 group, and the abundances demonstrated strong positive correlations between dam and offspring. These preliminary findings indicate that maternal opioid treatment during pregnancy alters the composition of the maternal microbiome, and this opioid-induced shift is similarly observed in offspring which could contribute to the impaired developmental phenotypes previously described.
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