MRI detection of impairment of glymphatic function in rat after mild traumatic brain injury.
MRI detection of impairment of glymphatic function in rat after mild traumatic brain injury.
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轻度颅脑损伤后大鼠淋巴功能损害的MRI检测
DOI：
10.1016/j.brainres.2020.147062
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发表时间：
2020-11-15
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
我们研究了轻度创伤性脑损伤（mTBI）对胶质淋巴通路的影响，使用对比增强磁共振成像（CE-MRI）和定量动力学参数从一个先进的二室模型。使用闭合头部撞击在雄性Wistar大鼠中诱导mTBI。有和没有mTBI的动物（n = 7/组）在损伤后10周接受相同的MRI方案，包括T2加权成像和3D T1加权成像，枕大池内注射造影剂（Gd-DTPA）。在多个脑组织区域中量化了表征活体脑中胶质淋巴示踪剂转运的动力学特征的输注速率、清除速率和清除时间常数的参数。在大多数检查区域中，我们的定量显示与健康对照相比，mTBI动物的输注和清除率显著降低，清除时间常数显著增加。这些数据表明，mTBI诱导造影剂流入和流出的慢性变化和胶质淋巴通路功能障碍。虽然mTBI后胶质淋巴功能的效率降低在脑中是明显的，但区域评估揭示了mTBI在不同解剖区域中的异质胶质淋巴效应。胶质淋巴功能的抑制，而不是局灶性病变的存在，表明mTBI后大脑的持续损伤。因此，动态CE-MRI结合先进的动力学分析，可提供一个有用的方法，客观评估和确诊mTBI。
We investigated the effect of mild traumatic brain injury (mTBI) on the glymphatic pathway using contrast-enhanced magnetic resonance imaging (CE-MRI) and quantified with kinetic parameters obtained from an advanced two-compartment model. mTBI was induced in male Wistar rats using a closed head impact. Animals with and without mTBI (n = 7/group) underwent the identical MRI protocol 10-weeks post-injury, including T2-weighted imaging and 3D T1-weighted imaging with intra-cisterna magna injection of contrast agent (Gd-DTPA). The parameters of infusion rate, clearance rate and clearance time constant, characterizing the kinetic features of glymphatic tracer transport in a living brain, were quantified in multiple brain tissue regions. In the majority of examined regions, our quantification demonstrated significantly reduced infusion and clearance rates, and significantly increased clearance time constant in the mTBI animals compared to the healthy controls. These data indicate that mTBI induces chronic changes in influx and efflux of contrast agent and glymphatic pathway dysfunction. While the reduced efficiency of glymphatic function after mTBI was apparent in brain, regional evaluation revealed heterogeneous glymphatic effects of the mTBI in different anatomical regions. The suppression of glymphatic function, rather than the presence of focal lesions, indicates a persistent injury of the brain after mTBI. Thus, dynamic CE-MRI in conjunction with advanced kinetic analysis may offer a useful methodology for an objective assessment and confirmatory diagnosis of mTBI.
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影响因子：
2.2
作者：
Dillard, Charles;Ditchman, Nicole;Neblett, Julie
通讯作者：
Neblett, Julie
影响因子：
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影响因子：
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