MRI investigation of glymphatic responses to Gd-DTPA infusion rates.
MRI investigation of glymphatic responses to Gd-DTPA infusion rates.
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DOI：
10.1002/jnr.24325
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发表时间：
2018-12
影响因子：
4.2
通讯作者：
Jiang Q
中科院分区：
文献类型：
--
作者：
Ding G;Chopp M;Li L;Zhang L;Davoodi-Bojd E;Li Q;Zhang Z;Jiang Q
胶质淋巴系统是一种新发现的大脑废物清除途径，主要使用体内双光子共聚焦显微镜发现和研究。与双光子共聚焦显微镜相比，磁共振成像（MRI）可提供全脑胶质淋巴系统的动态和真实的时间图像。我们采用MRI研究胶质淋巴系统对Gd-DTPA（magnetist）输注速率的反应。在MRI期间，对Wistar大鼠进行将管插入小脑延髓池中用于输注的手术。选择三种输注速率，将稀释的Magnevist输注到大鼠脑的脑脊液（CSF）中20 min。使用动态MRI 3D测量对淋巴反应成像5小时。在所有心室中发现图像增强的峰值强度与输注速率之间存在稳健的相关性，在第四心室中MRI图像增强的峰值时间与输注速率之间存在额外的相关性。以2.92 μL/min的速度注入，可使示踪剂在枕大池附近的第四脑室内明显蓄积。在海马组织中，图像增强表现出与输注速率的低相关性。然而，1.67 μL/min的输注速率提供了较高的图像增强，但枕大池附近的示踪剂积聚较少。对比增强MRI为研究大鼠脑胶质淋巴系统的图像对比度输注速率响应提供了合适的工具。考虑到对CSF中输注的磁降维素的T1和T2* 效应，1.67 μL/min的输注速率似乎适合于大鼠胶质淋巴系统的MRI研究。
The glymphatic system is a newly identified waste clearance pathway in brain discovered and investigated predominately using in vivo two‐photon confocal microscopy. Magnetic resonance imaging (MRI), in contrast to two‐photon confocal microscopy, provides dynamic and real‐time pictures of the glymphatic system in whole brain. We employ MRI to investigate the response of the glymphatic system to the rate of infusion of Gd‐DTPA (magnevist). Wistar rats were subjected to a surgery of inserting a tube into the cisterna magna for infusion during MRI. Three infusion rates were chosen for 20 min infusions of diluted magnevist into the cerebrospinal fluid (CSF) of rat brain. Glymphatic response was imaged using dynamic MRI 3D measurement for 5 hr. Robust correlations were found in all ventricles between the peak intensities of image enhancement and infusion rates, with additional correlations between the peak times of MRI image enhancement and infusion rates in the fourth ventricle. An infusion rate of 2.92 μL/min induced an evident accumulation of tracer in the fourth ventricle near the cisterna magna. In hippocampal tissue, image enhancements exhibited low correlation with the infusion rates. However, an infusion rate of 1.67 μL/min provided a high image enhancement, but less tracer accumulation near the cisterna magna. Contrast‐enhanced MRI provides a suitable tool for investigating image contrast infusion rate response of the glymphatic system in rat brain. Considering both T1 and T2* effects in response to the infused magnevist into CSF, the infusion rate of 1.67 μL/min appears suitable for MRI study of the glymphatic system in rat.
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影响因子：
7.7
作者：
Absinta M;Ha SK;Nair G;Sati P;Luciano NJ;Palisoc M;Louveau A;Zaghloul KA;Pittaluga S;Kipnis J;Reich DS
通讯作者：
Reich DS
影响因子：
12.7
作者：
Arbel-Ornath M;Hudry E;Eikermann-Haerter K;Hou S;Gregory JL;Zhao L;Betensky RA;Frosch MP;Greenberg SM;Bacskai BJ
通讯作者：
Bacskai BJ
DOI：
10.1177/0271678x17737984
发表时间：
2018-04
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
Bedussi B;Almasian M;de Vos J;VanBavel E;Bakker EN
通讯作者：
Bakker EN
影响因子：
2.9
作者：
RENNELS, ML;GREGORY, TF;GRADY, PA
通讯作者：
GRADY, PA
影响因子：
3.3
作者：
Lee, Hedok;Mortensen, Kristian;Benveniste, Helene
通讯作者：
Benveniste, Helene