Reconstitution of Listeria motility: implications for the mechanism of force transduction.
Reconstitution of Listeria motility: implications for the mechanism of force transduction.
复制标题
李斯特菌运动性的重建：对力传导机制的影响。
DOI：
10.1016/s0167-4889(99)00156-1
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Herzfeld,J
中科院分区：
文献类型：
--
作者：
Olbris,DJ;Herzfeld,J
单核细胞增生李斯特菌和其他一些感染性细菌将宿主细胞的肌动蛋白折叠成尾巴，推动细菌穿过细胞质。在这里，我们表明，这种行为在简单的介质中的重建解决了两个方面的力转导机制。首先，由于稀释的重组培养基没有细胞骨架，我们考虑是什么使尾巴不被向后推，而不是细菌被向前推。的依赖性的分割运动的摩擦系数的尾巴。与实验一致，我们发现，尾巴的运动阻力是敏感的长度。在完整的细胞中，即使是很小的尾巴也是静止不动的，这归因于锚定在细胞骨架上。第二，一些重构介质的相对低的粘度放大了扩散的效果，使得如果细菌和其尾部未附着，则在它们之间将形成大的间隙。在稀释的血小板提取物的粘度下，预测了几种细菌长度的稳态间隙。由于没有观察到这样的间隙，我们得出结论，李斯特菌必须附着在它们的尾巴上。我们考虑在生理条件下，这种依恋可能有什么目的。相关的病原体和变形虫运动的影响进行了讨论。
Listeria monocytogenes and some other infectious bacteria polymerize their host cell’s actin into tails that propel the bacteria through the cytoplasm. Here we show that reconstitution of this behavior in simpler media resolves two aspects of the mechanism of force transduction. First, since dilute reconstitution media have no cytoskeleton, we consider what keeps the tail from being pushed backward rather than the bacterium being propelled forward. The dependence of the partitioning of motion on the friction coefficient of the tail is derived. Consistent with experiments, we find that the resistance of the tail to motion is sensitive to its length. That even small tails are stationary in intact cells is attributed to anchoring to the cytoskeleton. Second, the comparatively low viscosity of some reconstitution media magnifies the effects of diffusion, such that a large gap will develop between the bacterium and its tail if they are unattached. At the viscosities of diluted platelet extracts, steady-state gaps of several bacterium lengths are predicted. Since such gaps are not observed, we conclude that Listeria must be attached to their tails. We consider what purposes such attachments might serve under physiological conditions. The implications for related pathogens and amoeboid locomotion are also discussed.
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